ArticleThe Journal of physiology1996
Actin microfilament disrupters enhance K(ATP) channel opening in patches from guinea-pig cardiomyocytes.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 104 citations in OpenAlex.
- Ion channels and actin: A tale of two friends.Histology and histopathology · 2025Review
- Channelling the Force to Reprogram the Matrix: Mechanosensitive Ion Channels in Cardiac Fibroblasts.Cells · 2021Review
- Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.Cell death & disease · 2019Article
- Leptin-induced Trafficking of KInternational journal of molecular sciences · 2019Review
- KATP Channels in the Cardiovascular System.Physiological reviews · 2016Review
- Electrical and Mechanical Strategies to Enable Cardiac Repair and Regeneration.IEEE reviews in biomedical engineering · 2015Review
- Article
- Compartmentation of membrane processes and nucleotide dynamics in diffusion-restricted cardiac cell microenvironment.Journal of molecular and cellular cardiology · 2012Review
- Role of endothelial nitric oxide in cerebrovascular regulation.Current pharmaceutical biotechnology · 2011Review
- ATP-sensitive potassium (KATP) channel activation decreases intraocular pressure in the anterior chamber of the eye.Investigative ophthalmology & visual science · 2011Article
- Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.Pflugers Archiv : European journal of physiology · 2011Review
- Differential roles for SUR subunits in KATP channel membrane targeting and regulation.American journal of physiology. Heart and circulatory physiology · 2011Article
- K(ATP) channels process nucleotide signals in muscle thermogenic response.Critical reviews in biochemistry and molecular biology · 2010Article
- Ankyrin-B regulates Kir6.2 membrane expression and function in heart.The Journal of biological chemistry · 2010Article
- Human K(ATP) channelopathies: diseases of metabolic homeostasis.Pflugers Archiv : European journal of physiology · 2010Review
- Dual role of K ATP channel C-terminal motif in membrane targeting and metabolic regulation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A dual mechanism of cytoprotection afforded by M-LDH in embryonic heart H9C2 cells.Biochimica et biophysica acta · 2009Article
- Impaired insulin exocytosis in neural cell adhesion molecule-/- mice due to defective reorganization of the submembrane F-actin network.Endocrinology · 2009Article
- Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Rho-kinase inhibition acutely augments blood flow in focal cerebral ischemia via endothelial mechanisms.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. To determine whether actin filament networks are associated with the regulation of ATP-sensitive K+ (K(ATP)) channel activity, single channel currents were measured in the inside-out configuration, and cytoskeletal disrupters applied to the internal side of patches excised from guinea-pig ventricular myocytes. 2. Treatment of patches with DNase I (10-200 micrograms ml(-1)), which forms complexes with G-actin and prevents actin filament formation, antagonized the ATP-induced inhibition of K(ATP) channels. 3. In the absence of ATP, DNase I did not increase K(ATP) channel activity. 4. When denatured by boiling or co-incubated with purified actin subunits (200 mu g ml(-1)), DNase I(100 mu g ml(-1)) did not antagonize the ATP-induced inhibition of K(ATP) channels. 5. The DNase I-induced decrease in the sensitivity of K(ATP) channels towards ATP-induced inhibition was partially restored by addition of purified actin subunits (200 micrograms ml(-1)). 6. Cytochalasin B (10 microM), another actin filament disrupter, but neither taxol nor nocodazole (30-100 microM), two antimicrotubule agents, enhanced K(ATP) channel activity in the presence of ATP. 7. Hence, actin filament disrupters can attenuate the ATP-dependent inhibitory gating of K(ATP) channels. This suggests that subsarcolemmal actin filament networks may be associated with the regulation of cardiac K(ATP) channels.
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