ArticleMolecular biology of the cell1997
Calcineurin-dependent nuclear translocation of a murine transcription factor NFATx: molecular cloning and functional characterization.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Role of NHERF1 in MicroRNA Landscape Changes in Aging Mouse Kidneys.Biomolecules · 2024Article
- Article
- NFAT2 Regulates Generation of Innate-Like CD8Frontiers in immunology · 2016Article
- NFAT4-dependent miR-324-5p regulates mitochondrial morphology and cardiomyocyte cell death by targeting Mtfr1.Cell death & disease · 2015Article
- Rapid selection of XO embryonic stem cells using Y chromosome-linked GFP transgenic mice.Transgenic research · 2014Article
- Estrogen receptor signaling and its relationship to cytokines in systemic lupus erythematosus.Journal of biomedicine & biotechnology · 2010Review
- 8-Bromo-cyclic inosine diphosphoribose: towards a selective cyclic ADP-ribose agonist.The Biochemical journal · 2009Article
- A tumor-suppressor function for NFATc3 in T-cell lymphomagenesis by murine leukemia virus.Blood · 2005Article
- Nuclear factor of activated T cells balances angiogenesis activation and inhibition.The Journal of experimental medicine · 2004Article
- Targeted disruption of NFATc3, but not NFATc4, reveals an intrinsic defect in calcineurin-mediated cardiac hypertrophic growth.Molecular and cellular biology · 2002Article
- Control of NFATx1 nuclear translocation by a calcineurin-regulated inhibitory domain.Molecular and cellular biology · 1997Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the nuclear factor of activated T cells (NFAT) are involved in the induction of a number of cytokine genes. We report here cDNA cloning and chromosomal localization of a murine homologue of human NFATx, designated as mNFATx1, and its splicing variants mNFATx2 and m delta NFATx. Northern blot analysis showed mNFATx1 to be predominantly expressed in the thymus. mNFATx1, but not m delta NFATx, produced in COS-7 cells, bound to all NFAT-binding sites of the interleukin (IL)-2 and IL-4 promoters tested. Immunofluorescence assay showed that both mNFATx1 and m delta NFATx introduced into COS-7 cells localized predominantly to the cytoplasm, but did translocate to the nucleus, either by cotransfection with an active form of calcineurin or wild-type calcineurin followed by stimulation with calcium ionophore. Translocation of mNFATx1 correlated well with activation of the murine IL-2 promoter; mNFATx1 translocated under conditions described above, in combination with phorbol 12-myristate 13-acetate, activated the transiently transfected murine IL-2 promoter. Thus, nuclear-translocated mNFATx1 is involved in activation of the IL-2 promoter. These results provide the first evidence for the requirement of calcineurin in the control of mNFATx imported from the cytoplasm to the nucleus and implies that mNFATx may possibly be a substrate of calcineurin in vivo.
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