ArticleMolecular biology of the cell1997
Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- IqgD is a Rac1-interacting IQGAP required for efficient growth of Dictyostelium discoideum on bacterial lawns.Cell communication and signaling : CCS · 2026Article
- The Dictyostelium discoideum FimA protein, unlike yeast and plant fimbrins, is regulated by calcium similar to mammalian plastins.Scientific reports · 2023Article
- Regulation of actin isoforms in cellular and developmental processes.Seminars in cell & developmental biology · 2020Review
- Interactome and F-Actin Interaction Analysis ofInternational journal of molecular sciences · 2020Article
- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
- Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis.The Journal of cell biology · 2017Article
- The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling.Scientific reports · 2017Article
- The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins.The Journal of biological chemistry · 2016Article
- Evidence of an evolutionarily conserved LMBR1 domain-containing protein that associates with endocytic cups and plays a role in cell migration in dictyostelium discoideum.Eukaryotic cell · 2012Article
- Cytokinesis through biochemical-mechanical feedback loops.Seminars in cell & developmental biology · 2010Review
- 14-3-3 coordinates microtubules, Rac, and myosin II to control cell mechanics and cytokinesis.Current biology : CB · 2010Article
- Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth.The Plant cell · 2010Article
- Single and multiple CH (calponin homology) domain containing multidomain proteins in Dictyostelium discoideum: an inventory.Molecular biology reports · 2010Article
- Propagating waves separate two states of actin organization in living cells.HFSP journal · 2009Article
- An active biopolymer network controlled by molecular motors.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions.The Journal of cell biology · 2008Article
- Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics.Current biology : CB · 2008Article
- Putting the brakes on cytokinesis with alpha-actinin.Developmental cell · 2007Article
- Myosin heavy-chain kinase A from Dictyostelium possesses a novel actin-binding domain that cross-links actin filaments.The Biochemical journal · 2006Article
- Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A protein purified from cytoskeletal fractions of Dictyostelium discoideum proved to be a member of the fimbrin/plastin family of actin-bundling proteins. Like other family members, this Ca(2+)-inhibited 67-kDa protein contains two EF hands followed by two actin-binding sites of the alpha-actinin/beta-spectrin type. Dd plastin interacted selectively with actin isoforms: it bound to D. discoideum actin and to beta/gamma-actin from bovine spleen but not to alpha-actin from rabbit skeletal muscle. Immunofluorescence labeling of growth phase cells showed accumulation of Dd plastin in cortical structures associated with cell surface extensions. In the elongated, streaming cells of the early aggregation stage, Dd plastin was enriched in the front regions. To examine how the bundled actin filaments behave in myosin II-driven motility, complexes of F-actin and Dd plastin were bound to immobilized heavy meromyosin, and motility was started by photoactivating caged ATP. Actin filaments were immediately propelled out of bundles or even larger aggregates and moved on the myosin as separate filaments. This result shows that myosin can disperse an actin network when it acts as a motor and sheds light on the dynamics of protein-protein interactions in the cortex of a motile cell where myosin II and Dd plastin are simultaneously present.
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