ArticleMolecular biology of the cell1997
Fibroblasts contracting collagen matrices form transient plasma membrane passages through which the cells take up fluorescein isothiocyanate-dextran and Ca2+.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Lysosome-Mediated Plasma Membrane Repair Is Dependent on the Small GTPase Arl8b and Determines Cell Death Type inJournal of immunology (Baltimore, Md. : 1950) · 2018Article
- Investigation of the spatiotemporal responses of nanoparticles in tumor tissues with a small-scale mathematical model.PloS one · 2013Article
- Neurite branch retraction is caused by a threshold-dependent mechanical impact.Biophysical journal · 2009Article
- RNA interference in human foreskin fibroblasts within the three-dimensional collagen matrix.Molecular and cellular biochemistry · 2007Article
- Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/Akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts.Molecular biology of the cell · 2004Article
- Impaired membrane resealing and autoimmune myositis in synaptotagmin VII-deficient mice.The Journal of cell biology · 2003Article
- Lysosomes and the plasma membrane: trypanosomes reveal a secret relationship.The Journal of cell biology · 2002Review
- Macromolecular uptake is a spontaneous event during mitosis in cultured fibroblasts: implications for vector-dependent plasmid transfection.Molecular biology of the cell · 2002Article
- Temporary disruption of the plasma membrane is required for c-fos expression in response to mechanical stress.Molecular biology of the cell · 1999Article
- The compliance of collagen gels regulates transforming growth factor-beta induction of alpha-smooth muscle actin in fibroblasts.The American journal of pathology · 1999Article
- Increased c-fos mRNA expression by human fibroblasts contracting stressed collagen matrices.Molecular and cellular biology · 1998Article
- Loss, restoration, and maintenance of plasma membrane integrity.The Journal of cell biology · 1997Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
When fibroblasts contract collagen matrices, the cells activate a Ca(2+)-dependent cyclic AMP signaling pathway. We have found that contraction also stimulates uptake of fluorescein isothiocyanate-dextran molecules from the medium. Our results indicate that fluorescein isothiocyanate-dextran enters directly into the cell cytoplasm through 3- to 5-nm plasma membrane passages. These passages, which reseal in less than 5 s in the presence of divalent cations, also are likely sites of Ca2+ uptake during contraction and the first step in contraction-activated cyclic AMP signaling. The formation of plasma membrane passages during fibroblast contraction may reflect a general cellular response to rapid mechanical changes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.