ArticleNucleic acids research1996
Functional domains of transcription factor hGABP beta1/E4TF1-53 required for nuclear localization and transcription activation.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- The immortality mechanism of TERT promoter mutant cancers is self-reinforcing and reversible.Molecular cell · 2025Article
- FGFR4 promotes nuclear localization of GABP to inhibit cell apoptosis in uterine leiomyosarcoma.Cell and tissue research · 2021Article
- GA binding protein augments autophagy via transcriptional activation of BECN1-PIK3C3 complex genes.Autophagy · 2014Article
- The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.Cell reports · 2013Article
- Targeting tetramer-forming GABPβ isoforms impairs self-renewal of hematopoietic and leukemic stem cells.Cell stem cell · 2012Article
- Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex.Molecular cancer · 2011Article
- Methylation-sensitive regulation of TMS1/ASC by the Ets factor, GA-binding protein-alpha.The Journal of biological chemistry · 2009Article
- Targeting the GA binding protein beta1L isoform does not perturb lymphocyte development and function.Molecular and cellular biology · 2008Article
- GA-binding protein is dispensable for neuromuscular synapse formation and synapse-specific gene expression.Molecular and cellular biology · 2007Article
- Characterization of a negative transcriptional element in the BRCA1 promoter.Breast cancer research : BCR · 2007Article
- The gene encoding the fragile X RNA-binding protein is controlled by nuclear respiratory factor 2 and the CREB family of transcription factors.Nucleic acids research · 2006Article
- Phosphorylation-elicited quaternary changes of GA binding protein in transcriptional activation.Molecular and cellular biology · 2003Article
- Regulation of E2F1-dependent gene transcription and apoptosis by the ETS-related transcription factor GABPgamma1.Molecular and cellular biology · 2002Article
- The Ets transcription factor GABP is required for postsynaptic differentiation in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Article
- Nuclear transport of the major capsid protein is essential for adeno-associated virus capsid formation.Journal of virology · 1999Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor E4TF1 is the human homolog of GABP and has been renamed hGABP (human GABP). hGABP is composed of two types of subunits; hGABP beta1/E4TF1-53 and the ets-related protein hGABP alpha/E4TF1-60. Both bind together to form an (alpha)2(beta1)2 heterotetrameric complex on DNA and activate transcription at specific promoters in vitro. Tetramer formation depends on two regions of hGABP beta1; the N-terminal region containing the Notch/ankyrin-type repeats is necessary for binding to hGABP alpha and the C-terminal region is necessary for homodimerization. In this report, we constructed various deletion mutants of hGABP beta1 in order to delimit the functional regions required for nuclear localization and transcription activity. We found that hGABP beta1 localization in the nucleus is dependent on a region located between amino acids 243 and 330 and that the presence of hGABP beta1 influences the efficiency of hGABP alpha transport into the nucleus. Next, we demonstrated that the hGABP complex composed of alpha and beta1 subunits activates transcription from the adenovirus early 4 promoter in vivo. This transcription activation needs the C-terminal region of hGABP beta1 and is consistent with results obtained with the in vitro assay. Furthermore, site-directed mutagenesis analysis of the C-terminal region reveals that the alpha-helix structure and the leucine residues are important for formation of a heterotetrameric complex with hGABP alpha in vitro and for transcription activation in vivo. These results suggest that hGABP beta1 stimulates transcription as part of a heterotetrameric complex with hGABP alpha in vivo.
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