ArticleNucleic acids research1996
The joining of non-complementary DNA double-strand breaks by mammalian extracts.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- CRISPR/Cas-based precision genome editing via microhomology-mediated end joining.Plant biotechnology journal · 2021Review
- Human RecQ Helicases in DNA Double-Strand Break Repair.Frontiers in cell and developmental biology · 2021Review
- Repair of DNA double-strand breaks by mammalian alternative end-joining pathways.The Journal of biological chemistry · 2018Review
- Article
- Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway?Trends in biochemical sciences · 2015Review
- Alternative end-joining mechanisms: a historical perspective.Frontiers in genetics · 2013Article
- The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining.Molecular and cellular biology · 2011Article
- Biochemical characterization of metnase's endonuclease activity and its role in NHEJ repair.Biochemistry · 2011Article
- Efficiency of nonhomologous DNA end joining varies among somatic tissues, despite similarity in mechanism.Cellular and molecular life sciences : CMLS · 2011Article
- Nonhomologous DNA end joining in cell-free extracts.Journal of nucleic acids · 2010Article
- TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1.BMC molecular biology · 2009Article
- Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology.Eukaryotic cell · 2007Article
- Article
- Article
- Processing of DNA for nonhomologous end-joining by cell-free extract.The EMBO journal · 2005Article
- DNA double strand break repair in human bladder cancer is error prone and involves microhomology-associated end-joining.Nucleic acids research · 2004Article
- Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences.Molecular and cellular biology · 2003Article
- Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome.The Plant cell · 2002Article
- In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts.Nucleic acids research · 2001Article
- The role of DNA polymerase activity in human non-homologous end joining.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a high efficiency system in which mammalian extracts join DNA double-strand breaks with non-complementary termini. This system has been used to obtain a large number of junction sequences from a range of different break-end combinations, allowing the elucidation of the joining mechanisms. Using an extract of calf thymus it was found that the major mechanism of joining was by blunt-end ligation following removal or fill-in of the single-stranded bases. However, some break-end combinations were joined through an efficient mechanism using short repeat sequences and we have succeeded in separating this mechanism from blunt-end joining by the biochemical fractionation of extracts. Characterization of activities and sequence data in an extensively purified fraction that will join ends by the repeat mechanism led to a model where joining is initiated by 3' strand invasion followed by pairing to short repeat sequences close to the break site. Thus the joining of double-strand breaks by mammalian extracts is achieved by several mechanisms and this system will allow the purification of the factors involved in each by the judicial choice of the non-complementary ends used in the assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.