ArticleNucleic acids research1996
In vivo persistence of DNA triple helices containing psoralen-conjugated oligodeoxyribonucleotides.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Evaluation of the public health laboratory network for tegumentary leishmaniasis in an endemic area of Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2024Article
- Repair of DNA lesions associated with triplex-forming oligonucleotides.Molecular carcinogenesis · 2009Review
- Sequence-specific triple helix formation with genomic DNA.Biochemistry · 2007Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- New insights into DNA triplexes: residual twist and radial difference as measures of base triplet non-isomorphism and their implication to sequence-dependent non-uniform DNA triplex.Nucleic acids research · 2005Article
- DNA binding and antigene activity of a daunomycin-conjugated triplex-forming oligonucleotide targeting the P2 promoter of the human c-myc gene.Nucleic acids research · 2004Article
- Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.Nucleic acids research · 2003Review
- Repair of triplex-directed DNA alkylation by nucleotide excision repair.Nucleic acids research · 2001Article
- Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking.Nucleic acids research · 2000Article
- Parallel intramolecular DNA triple helix with G and T bases in the third strand stabilized by Zn(2+) ions.Nucleic acids research · 2000Article
- Multiple rounds of transcription by RNA polymerase II at covalently cross-linked templates.Nucleic acids research · 1998Article
- Investigation of the formation and intracellular stability of purine.(purine/pyrimidine) triplexes.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triple helices represent an attractive method for modulating specific gene expression. In particular, cross-linking between a triplex-forming oligonucleotide (TFO) and its duplex DNA target, typically through the formation of psoralen photoadducts, allows efficient blocking of elongation by RNA polymerases in vitro. However, in vivo, this approach is limited by DNA repair of the photoadduct. Here we describe the use of an oligodeoxyribonucleotide 19mer psoralen-modified TFO to form covalent linkages between an oligonucleotide and both strands of the targeted duplex DNA, thereby efficiently blocking expression of a luciferase reporter gene. Most importantly, we demonstrate that both the psoralen cross-link and the purine-motif triplex remained intact for at least 72 h post-transfection, indicating that such species can persist for an extended period of time in vivo. These findings support the feasibility of an antigene approach for the therapeutic regulation of specific gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.