ArticleNucleic acids research1996
Variegated expression of a globin transgene correlates with chromatin accessibility but not methylation status.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Professor Emma Whitelaw, a pioneer of epigenetic inheritance.Frontiers in epigenetics and epigenomics · 2025Article
- Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase.Scientific reports · 2022Article
- miR-15a/16-1 deletion in activated B cells promotes plasma cell and mature B-cell neoplasms.Blood · 2021Article
- The use of mouse models to study epigenetics.Cold Spring Harbor perspectives in biology · 2013Review
- Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion.BMC physiology · 2012Article
- Isolation of undifferentiated and early differentiating type A spermatogonia from Pou5f1-GFP reporter mice.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Transcription-dependent silencing of inducible convergent transgenes in transgenic mice.Epigenetics & chromatin · 2010Article
- The molecular basis for stability of heterochromatin-mediated silencing in mammals.Epigenetics & chromatin · 2009Article
- Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice.The American journal of pathology · 2009Article
- Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development.Genome biology · 2008Article
- Article
- Mammalian linker-histone subtypes differentially affect gene expression in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Article
- Position effects are influenced by the orientation of a transgene with respect to flanking chromatin.Molecular and cellular biology · 2001Article
- Complex patterns of inheritance of an imprinted murine transgene suggest incomplete germline erasure.Nucleic acids research · 2000Article
- Enhancer-dependent transcriptional oscillations in mouse erythroleukemia cells.Molecular and cellular biology · 1999Article
- Cytosine methylation and mammalian development.Genes & development · 1999Article
- The locus control region is necessary for gene expression in the human beta-globin locus but not the maintenance of an open chromatin structure in erythroid cells.Molecular and cellular biology · 1998Article
- Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators.Genes & development · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are now many mammalian examples in which single cell assays of transgene activity have revealed variegated patterns of expression. We have previously reported that transgenes in which globin regulatory elements drive the lacZ reporter gene exhibit variegated expression patterns in mouse erythrocytes, with transgene activity detectable in only a sub-population of circulating erythroid cells. In order to elucidate the molecular mechanism responsible for variegated expression in this system, we have compared the chromatin structure and methylation status of the transgene locus in expressing and non-expressing populations of erythrocytes. We find that there is a difference in the chromatin conformation of the transgene locus between the two states. Relative to active transgenes, transgene loci which have been silenced exhibit a reduced sensitivity to general digestion by DNase I, as well as a failure to establish a transgene-specific DNase I hypersensitive site, suggesting that silenced transgenes are situated within less accessible chromatin structures. Surprisingly, the restrictive chromatin structure observed at silenced transgene loci did not correlate with increased methylation, with transgenes from both active and inactive loci appearing largely unmethylated following analysis with methylation-sensitive restriction enzymes and by sequencing PCR products derived from bisulphite-converted genomic DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.