ArticleNucleic acids research1996
Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 101 citations in OpenAlex.
- A plant tethering system for the functional study of protein-RNA interactions in vivo.Plant methods · 2022Article
- Review
- Molecular basis for AU-rich element recognition and dimerization by the HuR C-terminal RRM.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Post-transcriptional regulation of programmed cell death 4 (PDCD4) mRNA by the RNA-binding proteins human antigen R (HuR) and T-cell intracellular antigen 1 (TIA1).The Journal of biological chemistry · 2015Article
- Downregulation of β-actin and its regulatory gene HuR affect cell migration of human corneal fibroblasts.Molecular vision · 2014Article
- The C-terminal RNA binding motif of HuR is a multi-functional domain leading to HuR oligomerization and binding to U-rich RNA targets.RNA biology · 2014Article
- All three RNA recognition motifs and the hinge region of HuC play distinct roles in the regulation of alternative splicing.Nucleic acids research · 2013Article
- Mechanisms coordinating ELAV/Hu mRNA regulons.Current opinion in genetics & development · 2013Review
- Downregulation of β-actin gene and human antigen R in human keratoconus.Investigative ophthalmology & visual science · 2012Article
- Trans-acting factors governing acetylcholinesterase mRNA metabolism in neurons.Frontiers in molecular neuroscience · 2012Article
- Role of AUF1 and HuR in the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in LLC-PK₁-F⁺ cells.American journal of physiology. Renal physiology · 2011Article
- Novel recognition motifs and biological functions of the RNA-binding protein HuD revealed by genome-wide identification of its targets.Nucleic acids research · 2010Article
- Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3.Nucleic acids research · 2009Article
- The third RNA recognition motif of Drosophila ELAV protein has a role in multimerization.Nucleic acids research · 2008Article
- CARM1 regulates proliferation of PC12 cells by methylating HuD.Molecular and cellular biology · 2006Article
- ELAV multimerizes on conserved AU4-6 motifs important for ewg splicing regulation.Molecular and cellular biology · 2005Article
- Shared RNA-binding sites for interacting members of the Drosophila ELAV family of neuronal proteins.Nucleic acids research · 2005Article
- Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- ELAV inhibits 3'-end processing to promote neural splicing of ewg pre-mRNA.Genes & development · 2003Article
- A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG.The EMBO journal · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HuC is one of the RNA binding proteins which are suggested to play important roles in neuronal differentiation and maintenance. We cloned and sequenced cDNAs encoding a mouse protein which is homologous to human HuC (hHuC). The longest cDNA encodes a 367 amino acid protein with three RNA recognition motifs (RRMs) and displays 96% identity to hHuC. Northern blot analysis showed that two different mRNAs, of 5.3 and 4.3 kb, for mouse HuC (mHuC) are expressed specifically in brain tissue. Comparison of cDNA sequences with the corresponding genomic sequence revealed that alternative 3' splice site selection generates two closely related mHuC isoforms. Iterative in vitro RNA selection and binding analyses showed that both HuC isoforms can bind with almost identical specificity to sequences similar to the AU-rich element (ARE), which is involved in the regulation of mRNA stability. Functional domain mapping using mHuC deletion mutants showed that the first RRM binds to ARE, that the second RRM has no RNA binding activity by itself, but facilitates ARE binding by the first RRM and that the third RRM has specific binding activity for the poly(A) sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.