ArticleNucleic acids research1997
Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
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- An Epigenomic Roadmap Primes Non-Growing Oocytes for Maturation and Early Embryogenesis.bioRxiv : the preprint server for biology · 2025Article
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- NEAT1 enhances MPPImmunity, inflammation and disease · 2023Article
- Large-Scale Multi-Omics Studies Provide New Insights into Blood Pressure Regulation.International journal of molecular sciences · 2022Article
- Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development.Cell reports · 2022Article
- YAF2-Mediated YY1-Sirtuin6 Interactions Responsible for Mitochondrial Downregulation in Aging Tunicates.Molecular and cellular biology · 2021Article
- Cryptotanshinone interferes with chondrocyte apoptosis in osteoarthritis by inhibiting the expression of miR‑574‑5p.Molecular medicine reports · 2021Article
- The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability.International journal of molecular sciences · 2021Review
- Article
- Rybp orchestrates spermatogenesis via regulating meiosis and sperm motility in mice.Cell cycle (Georgetown, Tex.) · 2020Article
- Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription.Genes · 2019Review
- The polycomb group protein Yaf2 regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.The Journal of biological chemistry · 2018Article
- sFRP2 activates Wnt/β-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling.American journal of physiology. Cell physiology · 2016Article
- RYBP predicts survival of patients with non-small cell lung cancer and regulates tumor cell growth and the response to chemotherapy.Cancer letters · 2015Article
- Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts.American journal of physiology. Cell physiology · 2015Article
- YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment.Nucleic acids research · 2014Article
- Function of YY1 in Long-Distance DNA Interactions.Frontiers in immunology · 2014Review
- Generation and characterization of yeast two-hybrid cDNA libraries derived from two distinct mouse pluripotent cell types.Molecular biotechnology · 2013Article
- miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors.Orphanet journal of rare diseases · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muscle-restricted transcription of sarcomeric actin genes is negatively controlled by the zinc finger protein YY1, which is down-regulated at the protein level during myogenic differentiation. To identify cellular proteins that might mediate the function/stability of YY1 in muscle cells, we screened an adult human muscle cDNA library using the yeast two-hybrid cloning system. We report the isolation and characterization of a novel protein termed YAF2 (YY1- associated factor 2) that interacts with YY1. The YAF2 cDNA encodes a 180 amino acid basic protein (pI 10.5) containing a single N-terminal C2-X10-C2 zinc finger. Lysine clusters are present that may function as a nuclear localization signal. Domain mapping analysis shows that the first and second zinc fingers of YY1 are targeted for YAF2 protein interaction. In contrast to the down-regulation of YY1, YAF2 message levels increase during in vitro differentiation of both rat skeletal and cardiac muscle cells. YAF2 appears to have a promyogenic regulatory role, since overexpression of YAF2 in C2 myoblasts stimulates myogenic promoter activity normally restricted by YY1. Co-transfection of YY1 reverses the stimulatory effect of YAF2. YAF2 also greatly potentiates proteolytic cleavage of YY1 by the calcium- activated protease m-calpain. The isolation of YAF2 may help in understanding the mechanisms through which inhibitors of myogenic transcription may be antagonized or eliminated by proteolysis during muscle development.
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