ArticleNucleic acids research1997
The RAD5 gene product is involved in the avoidance of non-homologous end-joining of DNA double strand breaks in the yeast Saccharomyces cerevisiae.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Involvement of budding yeast Rad5 in translesion DNA synthesis through physical interaction with Rev1.Nucleic acids research · 2016Article
- A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae.BMC genomics · 2013Article
- RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana.The Plant cell · 2010Article
- Post-replication repair suppresses duplication-mediated genome instability.PLoS genetics · 2010Article
- A homolog of ScRAD5 is involved in DNA repair and homologous recombination in Arabidopsis.Plant physiology · 2008Article
- Postreplication repair and PCNA modification in Schizosaccharomyces pombe.Molecular biology of the cell · 2006Article
- Snf2 family ATPases and DExx box helicases: differences and unifying concepts from high-resolution crystal structures.Nucleic acids research · 2006Review
- The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent manner.Nucleic acids research · 2005Article
- A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.Nucleic acids research · 2002Article
- A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio.Genetics · 2002Article
- SIR functions are required for the toleration of an unrepaired double-strand break in a dispensable yeast chromosome.Molecular and cellular biology · 2001Article
- Article
- The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways.Genetics · 2000Article
- Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast.Nucleic acids research · 2000Article
- Repair of intermediate structures produced at DNA interstrand cross-links in Saccharomyces cerevisiae.Molecular and cellular biology · 2000Article
- Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1999Review
- Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo.Genetics · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In wild-type yeast, the repair of a 169 bp double-strand gap induced by the restriction enzymes ApaI and NcoI in the URA3gene of the shuttle vector YpJA18 occurs with high fidelity according to the homologous chromosomal sequence. In contrast, only 25% of the cells of rad5-7 and rad5 Delta mutants perform correct gap repair. As has been proven by sequencing of the junction sites, the remaining cells recircularise the gapped plasmids by joining of the non-compatible, non-homologous ends. Thus, regarding the repair of DNA double-strand breaks, the rad5 mutants behave like mammalian cells rather than budding yeast. The majority of the end joined plasmids miss either one or both of the 3'and 5'protruding single-strands of the restriction ends completely and have undergone blunt-end ligation accompanied by fill-in DNA synthesis. These results imply an important role for the Rad5 protein (Rad5p) in the protection of protruding single-strand ends and for the avoidance of non-homologous end joining during repair of double-strand gaps in budding yeast. Alternatively, the Rad5p may be an accessory factor increasing the efficiency of homologous recombination in yeast, however, the molecular mechanism of Rad5p function requires further investigation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.