ArticleNucleic acids research1997
Potassium-resistant triple helix formation and improved intracellular gene targeting by oligodeoxyribonucleotides containing 7-deazaxanthine.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 51 citations in OpenAlex.
- Liver-targeted gene therapy: Approaches and challenges.Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society · 2015Review
- Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases.Artificial DNA, PNA & XNA · 2014Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- Gene modulation for treating liver fibrosis.Critical reviews in therapeutic drug carrier systems · 2007Review
- Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides.Nucleic acids research · 2004Article
- Intracellular generation of single-stranded DNA for chromosomal triplex formation and induced recombination.Nucleic acids research · 2001Article
- Triplex formation by morpholino oligodeoxyribonucleotides in the HER-2/neu promoter requires the pyrimidine motif.Nucleic acids research · 2001Article
- Targeted inhibition of transcription elongation in cells mediated by triplex-forming oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Triple-helix formation induces recombination in mammalian cells via a nucleotide excision repair-dependent pathway.Molecular and cellular biology · 2000Article
- Thermodynamic and kinetic studies of the formation of triple helices between purine-rich deoxyribo-oligonucleotides and the promoter region of the human c-src proto-oncogene.Nucleic acids research · 1998Article
- Poly(L-lysine)-graft-dextran copolymer: amazing effects on triplex stabilization under physiological pH and ionic conditions (in vitro).Nucleic acids research · 1998Article
- Peptide nucleic acid-targeted mutagenesis of a chromosomal gene in mouse cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Triple helix formation by purine-rich oligonucleotides in the anti-parallel motif is inhibited by physiological concentrations of potassium. Substitution with 7-deazaxanthine (c7X) has been suggested as a strategy to overcome this effect. We have tested this by examining triple helix formation both in vitro and in vivo by a series of triple helix-forming oligonucleotides (TFOs) containing guanine plus either adenine, thymine, or c7X. The TFOs were conjugated to psoralen at the 5'end and were designed to bind to a portion of the supF mutation reporter gene. Using in vitro gel mobility shift assays, we found that triplex formation by the c7X-substituted TFOs was relatively resistant to the presence of 140 mM K+. The c7X-containing TFOs were also superior in gene targeting experiments in mammalian cells, yielding 4- to 5-fold higher mutation frequencies in a shuttle vector-based mutagenesis assay designed to detect mutations induced by third strand-directed psoralen adducts. When the phosphodiester backbone was replaced by a phosphorothioate one, the in vitro binding of the c7X-TFOs was not affected, but the efficiency of in vivo triple helix formation was reduced. These results indicate the utility of the c7X substitution for in vivo gene targeting experiments, and they show that the feasibility of the triplex anti-gene strategy can be significantly enhanced by advances in nucleotide chemistry.
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