ArticleNucleic acids research1997
Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS-PAGE and the size of the expressed protein is affected by the UTRs and sequences within the coding region of the CTCF gene.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Spatial expression of myocardin protein in normal and disease states.American journal of physiology. Cell physiology · 2026Article
- Direct analysis of transcription factor protected cfDNA in plasma by ChIP-seq: measurement of altered CTCF binding in cancer is a novel biomarker for liquid biopsy.Clinical epigenetics · 2026Article
- How the Intrinsically Disordered N-Terminus of Cancer/Testis Antigen MAGEA10 Is Responsible for Its Expression, Nuclear Localisation and Aberrant Migration.Biomolecules · 2023Article
- A novel gene, Le-Dd10, is involved in fruiting body formation of Lentinula edodes.Archives of microbiology · 2022Article
- HSF1 Can Prevent Inflammation following Heat Shock by Inhibiting the Excessive Activation of theCells · 2022Article
- StaphylococcalInternational journal of molecular sciences · 2022Article
- Defining the relative and combined contribution of CTCF and CTCFL to genomic regulation.Genome biology · 2020Article
- Developmental downregulation of LIS1 expression limits axonal extension and allows axon pruning.Biology open · 2017Article
- CCCTC-binding factor recruitment to the early region of the human papillomavirus 18 genome regulates viral oncogene expression.Journal of virology · 2015Article
- Tissue-specific insulator function at H19/Igf2 revealed by deletions at the imprinting control region.Human molecular genetics · 2014Article
- CCCTC-binding factor mediates effects of glucose on beta cell survival.Cell proliferation · 2014Article
- A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.Nucleic acids research · 2014Article
- CTCF mediates effect of insulin on glucagon expression.Experimental cell research · 2012Article
- Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase.BMC research notes · 2010Article
- CTCF terminal segments are unstructured.Protein science : a publication of the Protein Society · 2010Article
- A regulatory domain spanning the repeat sequence RE1 from herpes simplex virus type 1 has cell specific differential functions in trigeminal neurons and fibroblasts.FEBS letters · 2009Article
- The critical role of human transcriptional repressor CTCF mRNA up-regulation in the induction of anti-HIV-1 responses in CD4(+) T cells.Immunology letters · 2008Article
- Transcription factors CTCF and Pax6 are segregated to different cell types during retinal cell differentiation.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula.Plant physiology · 2007Article
- A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.Eukaryotic cell · 2007Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CTCF belongs to the Zn finger transcription factors family and binds to the promoter region of c-myc. CTCF is highly conserved between species, ubiquitous and localised in nuclei. The endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a 82 kDa protein (CTCF-82). In the present study we investigate this phenomenon and show with mass-spectra analysis that this occurs due to aberrant mobility of the CTCF protein. Another paradox is that our original cDNA, composed of the ORF and 3'-untranslated region (3'-UTR), produces a protein with the apparent molecular weight of 70 kDa (CTCF-70). This paradox has been found to be an effect of the UTRs and sequences within the coding region of the CTCF gene resulting in C-terminal truncation of CTCF-130. The potential attenuator has been identified and point-mutated. This restored the electrophoretic mobility of the CTCF protein to 130 kDa. CTCF-70, the aberrantly migrating CTCF N-terminus per se, is also detected in some cell types and therefore may have some biological implications. In particular, CTCF-70 interferes with CTCF-130 normal function, enhancing transactivation induced by CTCF-130 in COS6 cells. The mechanism of CTCF-70 action and other possible functions of CTCF-70 are discussed.
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