ArticleThe Journal of cell biology1997
Transplantation of quail collagen-tailed acetylcholinesterase molecules onto the frog neuromuscular synapse.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Perlecan, A Multi-Functional, Cell-Instructive, Matrix-Stabilizing Proteoglycan With Roles in Tissue Development Has Relevance to Connective Tissue Repair and Regeneration.Frontiers in cell and developmental biology · 2022Review
- Fine Localization of Acetylcholinesterase in the Synaptic Cleft of the Vertebrate Neuromuscular Junction.Frontiers in molecular neuroscience · 2018Article
- In Vitro Innervation as an Experimental Model to Study the Expression and Functions of Acetylcholinesterase and Agrin in Human Skeletal Muscle.Molecules (Basel, Switzerland) · 2017Review
- Biogenesis, assembly and trafficking of acetylcholinesterase.Journal of neurochemistry · 2017Review
- COOH-terminal collagen Q (COLQ) mutants causing human deficiency of endplate acetylcholinesterase impair the interaction of ColQ with proteins of the basal lamina.Human genetics · 2014Article
- Protein-anchoring strategy for delivering acetylcholinesterase to the neuromuscular junction.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Targeting of acetylcholinesterase in neurons in vivo: a dual processing function for the proline-rich membrane anchor subunit and the attachment domain on the catalytic subunit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Acetylcholinesterase expression in muscle is specifically controlled by a promoter-selective enhancesome in the first intron.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Acetylcholinesterase mobility and stability at the neuromuscular junction of living mice.Molecular biology of the cell · 2007Article
- MuSK is required for anchoring acetylcholinesterase at the neuromuscular junction.The Journal of cell biology · 2004Article
- Finite element simulations of acetylcholine diffusion in neuromuscular junctions.Biophysical journal · 2003Article
- Overloading and removal of N-glycosylation targets on human acetylcholinesterase: effects on glycan composition and circulatory residence time.The Biochemical journal · 2002Article
- The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane.The Journal of cell biology · 2001Article
- Local control of acetylcholinesterase gene expression in multinucleated skeletal muscle fibers: individual nuclei respond to signals from the overlying plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Stabilization of collagen-tailed acetylcholinesterase in muscle cells through extracellular anchorage by transglutaminase-catalyzed cross-linking.Molecular and cellular biochemistry · 2000Article
- Differences in expression of acetylcholinesterase and collagen Q control the distribution and oligomerization of the collagen-tailed forms in fast and slow muscles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Acetylcholinesterase clustering at the neuromuscular junction involves perlecan and dystroglycan.The Journal of cell biology · 1999Article
- Genetic analysis of collagen Q: roles in acetylcholinesterase and butyrylcholinesterase assembly and in synaptic structure and function.The Journal of cell biology · 1999Article
- Mutation in the human acetylcholinesterase-associated collagen gene, COLQ, is responsible for congenital myasthenic syndrome with end-plate acetylcholinesterase deficiency (Type Ic).American journal of human genetics · 1998Article
- Acetylcholinesterase mRNA level and synaptic activity in rat muscles depend on nerve-induced pattern of muscle activation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The highly organized pattern of acetylcholinesterase (AChE) molecules attached to the basal lamina of the neuromuscular junction (NMJ) suggests the existence of specific binding sites for their precise localization. To test this hypothesis we immunoaffinity purified quail globular and collagen-tailed AChE forms and determined their ability to attach to frog NMJs which had been pretreated with high-salt detergent buffers. The NMJs were visualized by labeling acetylcholine receptors (AChRs) with TRITC-alpha-bungarotoxin and AChE by indirect immunofluorescence; there was excellent correspondence (>97%) between the distribution of frog AChRs and AChE. Binding of the exogenous quail AChE was determined using a species-specific monoclonal antibody. When frog neuromuscular junctions were incubated with the globular G4/G2 quail AChE forms, there was no detectable binding above background levels, whereas when similar preparations were incubated with the collagen-tailed A12 AChE form >80% of the frog synaptic sites were also immunolabeled for quail AChE attached. Binding of the A12 quail AChE was blocked by heparin, yet could not be removed with high salt buffer containing detergent once attached. Similar results were obtained using empty myofiber basal lamina sheaths produced by mechanical or freeze-thaw damage. These experiments show that specific binding sites exist for collagen-tailed AChE molecules on the synaptic basal lamina of the vertebrate NMJ and suggest that these binding sites comprise a "molecular parking lot" in which the AChE molecules can be released, retained, and turned over.
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Registered trials
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