ArticleThe Journal of cell biology1997
A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Redox-dependent and redox-independent functions of Caenorhabditis elegans thioredoxin 1.Redox biology · 2019Article
- Functions and cellular compartmentation of the thioredoxin and glutathione pathways in yeast.Antioxidants & redox signaling · 2013Review
- Towards systematic discovery of signaling networks in budding yeast filamentous growth stress response using interventional phosphorylation data.PLoS computational biology · 2013Article
- SNARE function is not involved in early endosome docking.Molecular biology of the cell · 2008Article
- A Rab requirement is not bypassed in SLY1-20 suppression.Molecular biology of the cell · 2005Article
- Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast.Molecular biology of the cell · 2003Article
- Vacuole membrane fusion: V0 functions after trans-SNARE pairing and is coupled to the Ca2+-releasing channel.The Journal of cell biology · 2003Article
- Yeast vacuoles and membrane fusion pathways.The EMBO journal · 2002Review
- Genomic analysis of homotypic vacuole fusion.Molecular biology of the cell · 2002Article
- The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation.The EMBO journal · 2002Article
- The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum.Molecular biology of the cell · 2001Article
- Sequential action of two GTPases to promote vacuole docking and fusion.The EMBO journal · 2000Article
- A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.The EMBO journal · 2000Article
- Proteins needed for vesicle budding from the Golgi complex are also required for the docking step of homotypic vacuole fusion.The Journal of cell biology · 2000Article
- Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion.Molecular biology of the cell · 2000Article
- Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.Genetics · 2000Article
- Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae.Molecular biology of the cell · 1999Article
- Yeast VSM1 encodes a v-SNARE binding protein that may act as a negative regulator of constitutive exocytosis.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early in S phase, the vacuole (lysosome) of Saccharomyces cerevisiae projects a stream of vesicles and membranous tubules into the bud where they fuse and establish the daughter vacuole. This inheritance reaction can be studied in vitro with isolated vacuoles. Rapid and efficient homotypic fusion between salt-washed vacuoles requires the addition of only two purified soluble proteins, Sec18p (NSF) and LMA1, a novel heterodimer with a thioredoxin subunit. We now report the identity of the second subunit of LMA1 as I(B)2, a previously identified cytosolic inhibitor of vacuolar proteinase B. Both subunits are needed for efficient vacuole inheritance in vivo and for the LMA1 activity in cell extracts. Each subunit acts via a novel mechanism, as the thioredoxin subunit is not acting through redox chemistry and LMA1 is still needed for the fusion of vacuoles which do not contain proteinase B. Both Sec18p and LMA1 act at an early stage of the in vitro reaction. Though LMA1 does not stimulate Sec18p-mediated Sec17p release, LMA1 cannot fulfill its function before Sec18p. Upon Sec17p/Sec18p action, vacuoles become labile but are rapidly stabilized by LMA1. The action of LMA1 and Sec18p is thus coupled and ordered. These data establish LMA1 as a novel factor in trafficking of yeast vacuoles.
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