ArticleThe Plant cell1997
Phosphorylation of Opaque2 changes diurnally and impacts its DNA binding activity.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 96 citations in OpenAlex.
- Subclass III SnRK2 Kinases Coordinate Starch and Storage Protein Synthesis During Maize Kernel Development.Plant biotechnology journal · 2026Article
- Novel chromosome segment substitution lines derived fromBreeding science · 2025Article
- A pervasive phosphorylation cascade modulation of plant transcription factors in response to abiotic stress.Planta · 2023Review
- Wheat DOF transcription factors TaSAD and WPBF regulate glutenin gene expression in cooperation with SPA.PloS one · 2023Article
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- ABA-induced phosphorylation of basic leucine zipper 29, ABSCISIC ACID INSENSITIVE 19, and Opaque2 by SnRK2.2 enhances gene transactivation for endosperm filling in maize.The Plant cell · 2022Article
- Post-translational Modifications of bZIP Transcription Factors in Abscisic Acid Signaling and Drought Responses.Current genomics · 2021Review
- A SnRK1-The Plant cell · 2020Article
- Omics Data Reveal Putative Regulators of Einkorn Grain Protein Composition under Sulfur Deficiency.Plant physiology · 2020Article
- Systematic Analysis of Differentially Expressed MaizeInternational journal of molecular sciences · 2019Article
- The ZmbZIP22 Transcription Factor Regulates 27-kD γ-Zein Gene Transcription during Maize Endosperm Development.The Plant cell · 2018Article
- Identification and characterization of finger millet3 Biotech · 2018Article
- Phosphorylation Affects DNA-Binding of the Senescence-Regulating bZIP Transcription Factor GBF1.Plants (Basel, Switzerland) · 2015Article
- Emerging roles of protein kinase CK2 in abscisic acid signaling.Frontiers in plant science · 2015Review
- Fairy "tails": flexibility and function of intrinsically disordered extensions in the photosynthetic world.Frontiers in molecular biosciences · 2015Review
- Conserved cis-regulatory modules in promoters of genes encoding wheat high-molecular-weight glutenin subunits.Frontiers in plant science · 2014Article
- Reconstruction of protein networks from an atlas of maize seed proteotypes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Multifarious roles of intrinsic disorder in proteins illustrate its broad impact on plant biology.The Plant cell · 2013Review
- Zea mays Taxilin protein negatively regulates opaque-2 transcriptional activity by causing a change in its sub-cellular distribution.PloS one · 2012Article
- The thick aleurone1 mutant defines a negative regulation of maize aleurone cell fate that functions downstream of defective kernel1.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
In the maize endosperm, the Opaque2 (O2) basic leucine zipper transcriptional activator regulates the expression of a subset of the zein seed storage protein gene family. Immunodetection of wild-type or mutant O2 polypeptides fractionated by SDS-PAGE resolved a closely spaced doublet migrating in the 68- to 72-kD range, whereas by using isoelectric focusing, seven to nine isoforms were detected for each allele. Phosphatase treatment simplified the protein patterns to a single band corresponding to the nonphosphorylated component. In vivo and in vitro labeling confirmed that O2 can be phosphorylated. In protein gel blots probed with DNA, only the nonphosphorylated and hypophosphorylated O2 polypeptides were able to bind an oligonucleotide containing the O2 binding sequence. Upon in situ dephosphorylation of the focused isoforms by phosphatase treatment of the isoelectric focusing filter, the hyperphosphorylated forms acquired DNA binding activity. The ratio among the various isoforms remained constant throughout the developmental stages of endosperm growth but changed from daytime to nighttime, with a significant increase of the hyperphosphorylated forms during the night period. These results indicate that O2 exists in vivo as a pool of differently phosphorylated polypeptides and demonstrate that O2 DNA binding activity is modulated by a phosphorylation/dephosphorylation mechanism that appears to be influenced by environmental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.