ArticleProceedings of the National Academy of Sciences of the United States of America1997
Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- GREEN FLUORESCENT PROTEIN variants with enhanced folding are more efficiently imported into chloroplasts.Plant physiology · 2022Article
- Cytosolic Quality Control of Mitochondrial Protein Precursors-The Early Stages of the Organelle Biogenesis.International journal of molecular sciences · 2021Review
- Protein import in mitochondria biogenesis: guided by targeting signals and sustained by dedicated chaperones.RSC advances · 2021Review
- Review
- Role of the membrane potential in mitochondrial protein unfolding and import.Scientific reports · 2019Article
- Structural characterisation of a full-length mitochondrial outer membrane TOM40 preprotein translocase: implications for its interaction with presequence peptides.European biophysics journal : EBJ · 2019Article
- Article
- Article
- Protein Translocation into the Intermembrane Space and Matrix of Mitochondria: Mechanisms and Driving Forces.Frontiers in molecular biosciences · 2017Review
- Therapeutic applications of an expanded genetic code.Chembiochem : a European journal of chemical biology · 2014Review
- Direct interaction of mitochondrial targeting presequences with purified components of the TIM23 protein complex.The Journal of biological chemistry · 2011Article
- Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Tim23-Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import.The Journal of cell biology · 2009Article
- The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import.Molecular biology of the cell · 2007Article
- In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system.Nucleic acids research · 2006Article
- Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex.The EMBO journal · 2003Article
- Tom40, the import channel of the mitochondrial outer membrane, plays an active role in sorting imported proteins.The EMBO journal · 2003Article
- Tim50, a novel component of the TIM23 preprotein translocase of mitochondria.The EMBO journal · 2003Article
- Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The protein import motor of mitochondria: a targeted molecular ratchet driving unfolding and translocation.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Artificially aminoacylated suppressor tRNAs were used to introduce photoreactive amino acids into model mitochondrial precursor proteins to probe the environment along the protein import pathway. Amino acids with benzophenone side chains of various lengths [DL-2-amino-3-(p-benzoylphenyl)propanoic acid (1) and DL-2-amino-5-(p-benzoylphenyl)pentanoic acid (2)] were incorporated at specific sites throughout the cytochrome b2-dihydrofolate reductase fusion proteins, pb2(220)-DHFR and pb2 delta 19(220)-DHFR, which were destined for the intermembrane space and the matrix in mitochondria, respectively. In vitro import of pb2(220)-DHFR and pb2 delta 19(220)-DHFR bearing 1 or 2 into isolated yeast mitochondria was arrested so that the N terminus reached the intermembrane space or the matrix, respectively, while the DHFR domain remained at the mitochondrial surface. The matrix-targeted pb2 delta 19(220)-DHFR was photocrosslinked to Tom40 in the outer membrane, Tim44 in the inner membrane, and Ssc1p in the matrix, suggesting that the protein has an extended conformation in the import channels. On the other hand, incorporation of 2 at various positions in the 50-residue segment of intermembrane-space-targeted pb2(220)-DHFR gave photocrosslinks only to Tom40, suggesting that the segment is not in an extended conformation, but localized near Tom40. The N-terminal portion of pb2(220)-DHFR, but not pb2 delta 19(220)-DHFR, was photocrosslinked to an as-yet-unidentified mitochondrial component to generate a 95-kDa crosslinked product.
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Registered trials
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