ArticleProceedings of the National Academy of Sciences of the United States of America1997
Activities of human recombination protein Rad51.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 255 citations in OpenAlex.
- Machine Learning-Guided Identification of Madecassoside as a Potential RAD51 Modulator That Sensitizes Breast Cancer Cells to Cisplatin.Chemical biology & drug design · 2026Article
- Benzoxaborole-Based Inhibitors Block LexA Autocleavage and Suppress SOS-Dependent Adaptive Phenotypes inAntibiotics (Basel, Switzerland) · 2026Article
- SARS-CoV-2 exploits cellular RAD51 to promote viral propagation: implication of RAD51 inhibitor as a potential drug candidate against COVID-19.Journal of virology · 2023Article
- Small Molecules Targeting DNA Polymerase Theta (POLθ) as Promising Synthetic Lethal Agents for Precision Cancer Therapy.Journal of medicinal chemistry · 2023Review
- DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51.Nature communications · 2020Article
- Correction of the aprt Gene Using Repair-Polypurine Reverse Hoogsteen Hairpins in Mammalian Cells.Molecular therapy. Nucleic acids · 2020Article
- Clinicopathological and Prognostic Characteristics of RAD51 in Colorectal Cancer.Medicina (Kaunas, Lithuania) · 2020Article
- Gene Correction of Point Mutations Using PolyPurine Reverse Hoogsteen Hairpins Technology.Frontiers in genome editing · 2020Review
- Nonstructural Protein 5A Impairs DNA Damage Repair: Implications for Hepatitis C Virus-Mediated Hepatocarcinogenesis.Journal of virology · 2018Article
- Diverse role of survival motor neuron protein.Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange.Nature structural & molecular biology · 2017Article
- The protein PprI provides protection against radiation injury in human and mouse cells.Scientific reports · 2016Article
- ATP half-sites in RadA and RAD51 recombinases bind nucleotides.FEBS open bio · 2016Article
- β-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.PLoS pathogens · 2015Article
- DNA-pairing and annealing processes in homologous recombination and homology-directed repair.Cold Spring Harbor perspectives in biology · 2015Review
- Loop L1 governs the DNA-binding specificity and order for RecA-catalyzed reactions in homologous recombination and DNA repair.Nucleic acids research · 2015Article
- DNA strand exchange and RecA homologs in meiosis.Cold Spring Harbor perspectives in biology · 2014Review
- Repair of single-point mutations by polypurine reverse Hoogsteen hairpins.Human gene therapy methods · 2014Article
- The third exon of the budding yeast meiotic recombination gene HOP2 is required for calcium-dependent and recombinase Dmc1-specific stimulation of homologous strand assimilation.The Journal of biological chemistry · 2014Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Homologous pairing and strand exchange, which are catalyzed by Escherichia coli RecA protein, are central to homologous recombination. Homologs of this protein are found in eukaryotes; however, little has been reported on the recombinase activities of the mammalian homologs, including the human protein, denoted HsRad51. For the studies described here, we purified HsRad51 form E. coli. Although the activities of HsRad51 and RecA were qualitatively similar in the presence of ATP, there were also striking differences. The stoichiometry of binding to DNA and the rate of renaturation of complementary strands were similar for the two proteins, but rates of ATP hydrolysis, homologous pairing, and subsequent strand exchange promoted by HsRad51 were less than 1/10 those of RecA. In addition, HsRad51 bound gamma-thio-ATP and formed stable presynaptic complexes that promoted renaturation as rapidly as RecA, but the recombinant human protein catalyzed neither strand exchange nor homologous pairing of a single strand with duplex DNA in the presence of the ATP analog. By contrast, RecA promoted both of the latter reactions in control experiments. These observations suggest that among RecA-like proteins, HsRad51 may be a variant in which homologous pairing and strand exchange are more closely linked to the hydrolysis of ATP.
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