ArticleProceedings of the National Academy of Sciences of the United States of America1997
Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 192 citations in OpenAlex.
- Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family.Scientific reports · 2021Article
- Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction.Nucleus (Austin, Tex.) · 2019Article
- Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.Journal of proteome research · 2019Article
- The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing.The Plant cell · 2018Article
- Article
- RocSampler: regularizing overlapping protein complexes in protein-protein interaction networks.BMC bioinformatics · 2017Article
- SMN - A chaperone for nuclear RNP social occasions?RNA biology · 2017Review
- Active 5' splice sites regulate the biogenesis efficiency of Arabidopsis microRNAs derived from intron-containing genes.Nucleic acids research · 2017Article
- Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure.Scientific reports · 2016Article
- Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method.Journal of visualized experiments : JoVE · 2016Article
- Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae.RNA biology · 2016Article
- Article
- Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae.PLoS genetics · 2014Article
- A method for predicting protein-protein interaction types.PloS one · 2014Article
- Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Eukaryotic cell · 2013Article
- QTL analysis of high thermotolerance with superior and downgraded parental yeast strains reveals new minor QTLs and converges on novel causative alleles involved in RNA processing.PLoS genetics · 2013Article
- The proteomics big challenge for biomarkers and new drug-targets discovery.International journal of molecular sciences · 2012Review
- Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs.Nucleic acids research · 2012Article
- Cotranscriptional spliceosome assembly and splicing are independent of the Prp40p WW domain.RNA (New York, N.Y.) · 2011Article
- Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.Nucleic acids research · 2011Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report the rapid identification of the proteins of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) from the yeast Saccharomyces cerevisiae by searching mass spectrometric data in genomic sequence databases. The U1 snRNP, containing a histidine-tagged 70K protein, was isolated from cell extracts by anti m3G-cap immunoaffinity and subsequent nickel nitrilotriacetic acid chromatography. A U1 snRNP fraction containing 20 proteins was obtained. Further purification by glycerol gradient centrifugation identified nine U1 snRNP specific and six common proteins. The U1 snRNP proteins were partially sequenced by nanoelectrospray mass spectrometry, and their genes were identified in the data base via multiple peptide sequence tags. Apart from the already known common proteins D1, D3, F, and G, the D2 and E homologs were also identified. The same six common proteins were detected in core U2 snRNP, which was purified and analyzed separately. The biochemical association of these six proteins with yeast snRNPs is shown here for the first time. Intriguingly, the Sm B/B' homolog was not detected. In addition to the well characterized yeast U1 specific proteins [U1-70K (Snp1p), U1-A (Mud1p), Prp39p, and Prp40p] the homolog of the U1-C protein was identified together with four additional novel U1 specific proteins, which are not found in mammalian U1. This is the first time that the components of a multiprotein complex from an organism with a sequenced genome have been characterized by mass spectrometry. The technique should be applicable to any protein complex that can be biochemically purified from an organism whose genome is known.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.