ArticleThe Journal of cell biology1997
Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
88 citing papers in PubMed, 241 citations in OpenAlex.
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- Regulation of the Ras-Related Signaling Pathway by Small Molecules Containing an Indole Core Scaffold: A Potential Antitumor Therapy.Frontiers in pharmacology · 2020Review
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- HIV-1 transcription is regulated by splicing factor SRSF1.Nucleic acids research · 2014Article
- RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.Chemical reviews · 2013Review
- Cotranscriptional splicing efficiency differs dramatically between Drosophila and mouse.RNA (New York, N.Y.) · 2012Article
- Proteomic analysis of mitotic RNA polymerase II reveals novel interactors and association with proteins dysfunctional in disease.Molecular & cellular proteomics : MCP · 2012Article
- TCERG1 regulates alternative splicing of the Bcl-x gene by modulating the rate of RNA polymerase II transcription.Molecular and cellular biology · 2012Article
- Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.The Journal of cell biology · 2011Article
- Analogues and derivatives of oncrasin-1, a novel inhibitor of the C-terminal domain of RNA polymerase II and their antitumor activities.Journal of medicinal chemistry · 2011Article
- The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing.PLoS biology · 2011Article
- Updating the CTD Story: From Tail to Epic.Genetics research international · 2011Article
- Spatial Organization and Dynamics of Transcription Elongation and Pre-mRNA Processing in Live Cells.Genetics research international · 2011Article
- Article
- Differential effects of sumoylation on transcription and alternative splicing by transcription elongation regulator 1 (TCERG1).The Journal of biological chemistry · 2010Article
- Efficient transcription through an intron requires the binding of an Sm-type U1 snRNP with intact stem loop II to the splice donor.Nucleic acids research · 2010Article
- New insights into the biogenesis of nuclear RNA polymerases?Biochemistry and cell biology = Biochimie et biologie cellulaire · 2010Review
- Repression of zygotic gene expression in the Xenopus germline.Development (Cambridge, England) · 2010Article
- Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7.The Journal of biological chemistry · 2009Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) contains multiple tandem copies of the consensus heptapeptide, TyrSerProThrSerProSer. Concomitant with transcription initiation the CTD is phosphorylated. Elongating polymerase has a hyperphosphorylated CTD, but the role of this modification is poorly understood. A recent study revealed that some hyperphosphorylated polymerase molecules (Pol IIo) are nonchromosomal, and hence transcriptionally unengaged (Bregman, D.B., L. Du, S. van der Zee, S.L. Warren. 1995. J. Cell Biol. 129: 287-298). Pol IIo was concentrated in discrete splicing factor domains, suggesting a possible relationship between CTD phosphorylation and splicing factors, but no evidence beyond immunolocalization data was provided to support this idea. Here, we show that Pol IIo co-immunoprecipitates with members of two classes of splicing factors, the Sm snRNPs and non-snRNP SerArg (SR) family proteins. Significantly, Pol IIo's association with splicing factors is maintained in the absence of pre-mRNA, and the polymerase need not be transcriptionally engaged. We also provide definitive evidence that hyperphosphorylation of Pol II's CTD is poorly correlated with its transcriptional activity. Using monoclonal antibodies (mAbs) H5 and H14, which are shown here to recognize phosphoepitopes on Pol II's CTD, we have quantitated the level of Pol IIo at different stages of the cell cycle. The level of Pol IIo is similar in interphase and mitotic cells, which are transcriptionally active and inactive, respectively. Finally, complexes containing Pol IIo and splicing factors can be prepared from mitotic as well as interphase cells. The experiments reported here establish that hyperphosphorylation of the CTD is a good indicator of polymerase's association with snRNP and SR splicing factors, but not of its transcriptional activity. Most importantly, the present study suggests that splicing factors may associate with the polymerase via the hyperphosphorylated CTD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.