ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Retrograde transport and steady-state distribution of 125I-nerve growth factor in rat sympathetic neurons in compartmented cultures.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 119 citations in OpenAlex.
- Spatial and temporal aspects of muscle hyperalgesia induced by nerve growth factor in humans.Experimental brain research · 2008Trial
- NGF Signaling in Endosomes.Advances in experimental medicine and biology · 2021Article
- The many disguises of the signalling endosome.FEBS letters · 2018Review
- Control of neurite growth and guidance by an inhibitory cell-body signal.PLoS computational biology · 2018Article
- PROneurotrophins and CONSequences.Molecular neurobiology · 2018Review
- Phospho-Regulation of Soma-to-Axon Transcytosis of Neurotrophin Receptors.Developmental cell · 2017Article
- Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands.Nature communications · 2016Article
- Single-particle tracking uncovers dynamics of glutamate-induced retrograde transport of NF-κB p65 in living neurons.Neurophotonics · 2016Article
- Neurotrophin signaling endosomes: biogenesis, regulation, and functions.Current opinion in neurobiology · 2016Review
- Visualizing directional Rab7 and TrkA cotrafficking in axons by pTIRF microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Production and isolation of axons from sensory neurons for biochemical analysis using porous filters.Journal of visualized experiments : JoVE · 2014Article
- Axon myelination and electrical stimulation in a microfluidic, compartmentalized cell culture platform.Neuromolecular medicine · 2012Article
- The role of mesopontine NGF in sleep and wakefulness.Brain research · 2011Article
- Regulation of trafficking of activated TrkA is critical for NGF-mediated functions.Traffic (Copenhagen, Denmark) · 2011Article
- Single-molecule imaging of NGF axonal transport in microfluidic devices.Lab on a chip · 2010Article
- Limited trafficking of a neurotropic virus through inefficient retrograde axonal transport and the type I interferon response.PLoS pathogens · 2010Article
- Tri-partite complex for axonal transport drug delivery achieves pharmacological effect.BMC neuroscience · 2010Article
- Fates of neurotrophins after retrograde axonal transport: phosphorylation of p75NTR is a sorting signal for delayed degradation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Production of compartmented cultures of rat sympathetic neurons.Nature protocols · 2009Article
- Preparation and maintenance of dorsal root ganglia neurons in compartmented cultures.Journal of visualized experiments : JoVE · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used compartmented cultures of rat sympathetic neurons to quantitatively examine the retrograde transport of 125I-nerve growth factor (NGF) supplied to distal axons and to characterize the cellular events that maintain steady-state levels of NGF in cell bodies. In cultures allowed to reach steady-state 125I-NGF transport, cell bodies contained only 5-30% of the total neuron-associated 125I-NGF, whereas 70-95% remained associated with the distal axons. This was true over an 8 pM to 1.5 nM 125I-NGF concentration range, indicating that saturation of high affinity receptors could not account for the large fraction of 125I-NGF remaining in axons. Dissociation assays indicated that 85% of 125I-NGF associated with distal axons was surface-bound. At steady-state, only 2-25% of the distal axon-associated 125I-NGF was retrogradely transported each hour, with higher transport rates associated with younger cultures and lower 125I-NGF concentrations. The velocity of 125I-NGF retrograde transport was estimated at 10-20 mm/hr. However, as in a previous report, almost no 125I-NGF transport was observed during the first hour after 125I-NGF administration, indicating a significant lag between receptor binding and loading onto the retrograde transport system. During 125I-NGF transport through axons spanning an intermediate compartment in five-compartment cultures, little or no 125I-NGF was degraded or released from the axons. After transport, 125I-NGF was degraded with a half-life of 3 hr. In summary, although some cellular events promoted NGF accumulation in cell bodies, distal axons represented by far the principal site of NGF-receptor interaction at steady-state as a result of a low retrograde transport rate.
Indexed as
Identifiers
9006972PMC6793719W2131388825What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.