ArticleThe American journal of pathology1997
Activation of matrix metalloproteinase-2 from hepatic stellate cells requires interactions with hepatocytes.
Article in The American journal of pathology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 97 citations in OpenAlex.
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- Hepatoprotective Potency of Chrysophanol 8-International journal of molecular sciences · 2020Article
- Mechanisms of fibrogenesis in liver cirrhosis: The molecular aspects of epithelial-mesenchymal transition.World journal of hepatology · 2014Review
- Hepatocyte-stellate cell cross-talk in the liver engenders a permissive inflammatory microenvironment that drives progression in hepatocellular carcinoma.Cancer research · 2012Article
- The vacuolar-ATPase modulates matrix metalloproteinase isoforms in human pancreatic cancer.Laboratory investigation; a journal of technical methods and pathology · 2011Article
- CX3CL1/fractalkine shedding by human hepatic stellate cells: contribution to chronic inflammation in the liver.Journal of cellular and molecular medicine · 2009Article
- Dual role of CCR2 in the constitution and the resolution of liver fibrosis in mice.The American journal of pathology · 2009Article
- Ethanol exposure depletes hepatic pigment epithelium-derived factor, a novel lipid regulator.Gastroenterology · 2009Article
- Liver repopulation and carcinogenesis: two sides of the same coin?The American journal of pathology · 2008Review
- Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver.Physiological reviews · 2008Review
- Interleukin-6 protects hepatocytes from CCl4-mediated necrosis and apoptosis in mice by reducing MMP-2 expression.Journal of hepatology · 2005Article
- DDR2 receptor promotes MMP-2-mediated proliferation and invasion by hepatic stellate cells.The Journal of clinical investigation · 2001Article
- Effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 in hepatic stellate cells.World journal of gastroenterology · 2001Article
- Immunohistochemical analysis of development of desmin-positive hepatic stellate cells in mouse liver.Journal of anatomy · 2000Article
- Altered balance between matrix metalloproteinases and their inhibitors in experimental biliary fibrosis.The American journal of pathology · 1998Article
- Differential expression and origin of membrane-type 1 and 2 matrix metalloproteinases (MT-MMPs) in association with MMP2 activation in injured human livers.The American journal of pathology · 1998Article
- Proteolytic processing of membrane-type-1 matrix metalloproteinase is associated with gelatinase A activation at the cell surface.The Biochemical journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of matrix metalloproteinase (MMP)-2, the 72-kd collagenase IV/gelatinase A, is involved in extracellular matrix remodeling. It has been suggested that a membrane-type MMP (MT-MMP-1) and the tissue inhibitor of metalloproteinase (TIMP)-2 are involved in MMP-2 processing, but the exact mechanism(s) of its activation remains unclear. We have investigated the role of cell-cell cooperation in the activation of pro-MMP-2 in the liver, using pure cultures and co-cultures of hepatocytes and hepatic stellate cells (HSCs). Northern blot analysis and in situ hybridization showed that, in both pure and co-cultures, HSCs, but not hepatocytes, expressed MMP-2, TIMP-2, and MT-MMP-1 mRNA. Zymography analyses revealed the latent form of MMP-2 in medium from 2-day-old pure HSC cultures with higher amounts in medium from hepatocyte/HSC co-cultures. When hepatocytes were added to 10-day-old HSC cultures, the activated form of MMP-2 was detected, concomitantly with the deposition of an abundant extracellular matrix. Incubation of plasma membrane-enriched fractions from hepatocytes with conditioned medium from pure HSC cultures generated the activated species of MMP-2 (62 and 59 kd). Activation of pro-MMP-2 by hepatocyte membranes was inhibited by EDTA, heat, and trypsin but not by serine proteinase inhibitors. These data show that the co-expression of TIMP-2, MMP-2, and MT-MMP-1 by HSCs does not lead to secretion of the activated form of MMP-2. Hepatocytes, which do not express MMP-2, TIMP-2, or MT-MMP-1, induce MMP-2 activation through a plasma membrane-dependent mechanism(s), thus suggesting that cell-cell interactions are involved in this process in vivo.
Indexed as
Identifiers
9006321PMC1858523W2309242976What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.