ArticleThe Journal of clinical investigation1997
Deficiency of Src family kinases Fgr and Hck results in activation of erythrocyte K/Cl cotransport.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 114 citations in OpenAlex.
- Repurposing Tyrosine Kinase Inhibitors for Sickle Cell Disease: Focus on Band 3 Phosphorylation.Biomedicines · 2026Review
- Targeting Lyn Kinase in Chorea-Acanthocytosis: A Translational Treatment Approach in a Rare Disease.Journal of personalized medicine · 2021Article
- Oxidation Impacts the Intracellular Signaling Machinery in Hematological Disorders.Antioxidants (Basel, Switzerland) · 2020Review
- Genetic disruption of KCC cotransporters in a mouse model of thalassemia intermedia.Blood cells, molecules & diseases · 2020Article
- The role of WNK in modulation of KCl cotransport activity in red cells from normal individuals and patients with sickle cell anaemia.Pflugers Archiv : European journal of physiology · 2019Article
- Combined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism.Scientific reports · 2018Article
- A new molecular link between defective autophagy and erythroid abnormalities in chorea-acanthocytosis.Blood · 2016Article
- Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Inhibition of an Erythrocyte Tyrosine Kinase with Imatinib Prevents Plasmodium falciparum Egress and Terminates Parasitemia.PloS one · 2016Article
- Red Blood Cell Homeostasis: Pharmacological Interventions to Explore Biochemical, Morphological and Mechanical Properties.Frontiers in molecular biosciences · 2016Article
- A novel regulatory locus of phosphorylation in the C terminus of the potassium chloride cotransporter KCC2 that interferes with N-ethylmaleimide or staurosporine-mediated activation.The Journal of biological chemistry · 2014Article
- Oxidative stress and β-thalassemic erythroid cells behind the molecular defect.Oxidative medicine and cellular longevity · 2013Review
- Functions of the Lyn tyrosine kinase in health and disease.Cell communication and signaling : CCS · 2012Article
- Peroxiredoxin-2 expression is increased in beta-thalassemic mouse red cells but is displaced from the membrane as a marker of oxidative stress.Free radical biology & medicine · 2010Article
- Regulation of K-Cl cotransport in erythrocytes of frog Rana temporaria by commonly used protein kinase and protein phosphatase inhibitors.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2010Article
- A novel erythroid anion exchange variant (Gly796Arg) of hereditary stomatocytosis associated with dyserythropoiesis.Haematologica · 2009Article
- Effects of phorbol 12-myristate 13-acetate on potassium transport in the red blood cells of frog Rana temporaria.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2009Article
- PTPepsilon has a critical role in signaling transduction pathways and phosphoprotein network topology in red cells.Proteomics · 2008Article
- Urea stimulation of KCl cotransport induces abnormal volume reduction in sickle reticulocytes.Blood · 2007Article
- Regulation of K-Cl cotransport by protein phosphatase 1alpha in mouse erythrocytes.Pflugers Archiv : European journal of physiology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Src-family kinases play a central role in regulation of hematopoietic cell functions. We found that mouse erythrocytes express the Src-family kinases Fgr and Hck, as well as Lyn. To directly test whether Fgr and Hck play any role in erythrocyte function, we analyzed red cells isolated from fgr-/-, hck-/-, and fgr-/- hck-/- knock-out mice. Mean corpuscular hemoglobin concentration and median density are increased, while K content is decreased, in fgr-/- hck-/- double-mutant erythrocytes compared with wild-type, fgr-/-, or hck-/- erythrocytes. Na/K pump and Na/K/Cl cotransport were not altered, but K/Cl cotransport activity was significantly and substantially higher (approximately three-fold) in fgr-/- hck-/- double-mutant erythrocytes. This enhanced K/Cl cotransport activity did not depend on cell age. In fact, in response to bleeding, K/Cl cotransport activity increased in parallel with reticulocytosis in wild-type erythrocytes, while abnormal K/Cl cotransport did not change as a consequence of reticulocytosis in fgr-/- hck-/- double-mutant erythrocytes. Okadaic acid, an inhibitor of a phosphatase that has been implicated in activation of the K/Cl cotransporter, inhibited K/Cl cotransport in wild-type and fgr-/- hck-/- double-mutant erythrocytes to a comparable extent. In contrast, staurosporine, an inhibitor of a kinase that has been suggested to negatively regulate this same phosphatase enhanced K/Cl cotransport in wild-type but not in fgr-/- hck-/- double-mutant erythrocytes. On the basis of these findings, we propose that Fgr and Hck are the kinases involved in the negative regulation of the K/Cl cotransporter-activating phosphatase. Abnormality of erythrocyte K/Cl cotransport in fgr-/- hck-/- double-mutant animals represents the first demonstration that Src-family kinases may be involved in regulation of membrane transport.
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Registered trials
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