ArticleThe EMBO journal1996
Polyadenylation accelerates degradation of chloroplast mRNA.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 127 citations in OpenAlex.
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- Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.Biomolecules · 2020Article
- RNA polyadenylation and its consequences in prokaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
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- Integration of plastids with their hosts: Lessons learned from dinoflagellates.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- Characterization of the polyadenylation activity in a replicase complex from Bamboo mosaic virus-infected Nicotiana benthamiana plants.Virology · 2013Article
- Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae.Plant molecular biology · 2012Article
- Overaccumulation of the chloroplast antisense RNA AS5 is correlated with decreased abundance of 5S rRNA in vivo and inefficient 5S rRNA maturation in vitro.RNA (New York, N.Y.) · 2011Article
- Transient expression of βC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions.Virology journal · 2010Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase.Journal of virology · 2008Article
- Heat stress enhances the accumulation of polyadenylated mitochondrial transcripts in Arabidopsis thaliana.PloS one · 2008Article
- Article
- Stable PNPase RNAi silencing: its effect on the processing and adenylation of human mitochondrial RNA.RNA (New York, N.Y.) · 2008Article
- Integration of chloroplast nucleic acid metabolism into the phosphate deprivation response in Chlamydomonas reinhardtii.The Plant cell · 2007Article
- Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway.Plant molecular biology · 2007Article
- Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of chloroplast genes is regulated by several mechanisms, one of which is the modulation of RNA stability. To understand how this regulatory step is controlled during chloroplast development, we have begun to define the mechanism of plastid mRNA degradation. We show here that the degradation petD mRNA involves endonucleolytic cleavage at specific sites upstream of the 3' stem-loop structure. The endonucleolytic petD cleavage products can be polyadenylated in vitro, and similar polyadenylated RNA products are detectable in vivo. PCR analysis of the psbA and psaA-psaB-rps14 operons revealed other polyadenylated endonucleolytic cleavage products, indicating that poly(A) addition appears to be an integral modification during chloroplast mRNA degradation. Polyadenylation promotes efficient degradation of the cleaved petD RNAs by a 3'-5' exoribonuclease. Furthermore, polyadenylation also plays an important role in the degradation of the petD mRNA 3' end. Although the 3' end stem-loop is usually resistant to nucleases, adenylation renders the secondary structure susceptible to the 3'-5' exoribonuclease. Analysis of 3' ends confirms that polyadenylation occurs in vivo, and reveals that the extent of adenylation increases during the degradation of plastid mRNA in the dark. Based on these results, we propose a novel mechanism for polyadenylation in the regulation of plastid mRNA degradation.
Indexed as
Identifiers
9003789PMC452540W2338979667What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.