ArticleThe EMBO journal1996
Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Building on-chip cytoskeletal circuits via branched microtubule networks.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
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- HepaCAM inhibits cell proliferation and invasion in prostate cancer by suppressing nuclear translocation of the androgen receptor via its cytoplasmic domain.Molecular medicine reports · 2019Article
- Dissecting Protein Complexes in Branching Microtubule Nucleation Using MeioticCold Spring Harbor protocols · 2018Article
- Enhanced Nucleocytoplasmic Transport due to Competition for Elastic Binding Sites.Biophysical journal · 2018Article
- Karyopherins regulate nuclear pore complex barrier and transport function.The Journal of cell biology · 2017Article
- Structural analysis of the role of TPX2 in branching microtubule nucleation.The Journal of cell biology · 2017Article
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- Letermovir and inhibitors of the terminase complex: a promising new class of investigational antiviral drugs against human cytomegalovirus.Infection and drug resistance · 2015Review
- TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts.The Journal of cell biology · 2014Article
- Nuclear translocation of proteins and the effect of phosphatidic acid.Plant signaling & behavior · 2014Article
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- Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation.Nature cell biology · 2012Article
- The importin β binding domain as a master regulator of nucleocytoplasmic transport.Biochimica et biophysica acta · 2011Review
- A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1.The Journal of biological chemistry · 2011Article
- The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex.The Journal of biological chemistry · 2010Article
- CaM kinase II initiates meiotic spindle depolymerization independently of APC/C activation.The Journal of cell biology · 2008Article
- Localized regulation of axonal RanGTPase controls retrograde injury signaling in peripheral nerve.Neuron · 2008Article
- Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rate.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The small nuclear GTP binding protein Ran is required for transport of nuclear proteins through the nuclear pore complex (NPC). Although it is known that GTP hydrolysis by Ran is essential for this reaction, it has been unclear whether additional energy-consuming steps are also required. To uncouple the energy requirements for Ran from other nucleoside triphosphatases, we constructed a mutant derivative of Ran that has an altered nucleotide specificity from GTP to xanthosine 5' triphosphate. Using this Ran mutant, we demonstrate that nucleotide hydrolysis by Ran is sufficient to promote efficient nuclear protein import in vitro. Under these conditions, protein import could no longer be inhibited with non-hydrolysable nucleotide analogues, indicating that no Ran-independent energy-requiring steps are essential for the protein translocation reaction through the NPC. We further provide evidence that nuclear protein import requires Ran in the GDP form in the cytoplasm. This suggests that a coordinated exchange reaction from Ran-GDP to Ran-GTP at the pore is necessary for translocation into the nucleus.
Indexed as
Identifiers
9003787PMC452537What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.