ArticleThe EMBO journal1996
Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 167 citations in OpenAlex.
- Cellular and molecular outcomes of Pseudomonas putida KT2440 exposure to aluminium.Folia microbiologica · 2025Article
- Revealing the Bacterial HslV Protease Activation Potential with Triazine Derivatives via Experimental and Computational Approaches.The protein journal · 2025Article
- A Transient π-π or Cation-π Interaction between Degron and Degrader Dual Residues: A Key Step for the Substrate Recognition and Discrimination in the Processive Degradation of SulA by ClpYQ (HslUV) Protease inInternational journal of molecular sciences · 2023Article
- Checkpoints That Regulate Balanced Biosynthesis of Lipopolysaccharide and Its Essentiality inInternational journal of molecular sciences · 2021Review
- Protein degradation control and regulation of bacterial survival and pathogenicity: the role of protein degradation systems in bacteria.Molecular biology reports · 2021Review
- The holobiont transcriptome of teneral tsetse fly species of varying vector competence.BMC genomics · 2021Article
- Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease.International journal of molecular sciences · 2020Article
- Genetic Mutations That Drive Evolutionary Rescue to Lethal Temperature in Escherichia coli.Genome biology and evolution · 2020Article
- Identification of Substrates of Cytoplasmic Peptidyl-ProlylInternational journal of molecular sciences · 2020Article
- The HslV Protease fromInternational journal of molecular sciences · 2019Article
- Escherichia coli Proteome Microarrays Identified the Substrates of ClpYQ Protease.Molecular & cellular proteomics : MCP · 2017Article
- High yields and soluble expression of superoxide dismutases in Escherichia coli due to the HIV-1 Tat peptide via increases in mRNA transcription.Experimental & molecular medicine · 2016Article
- Escherichia coli ClpB is a non-processive polypeptide translocase.The Biochemical journal · 2015Article
- Molecular Chaperones of Leishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes.BioMed research international · 2015Review
- Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.PloS one · 2014Article
- Sulfur-34S stable isotope labeling of amino acids for quantification (SULAQ34) of proteomic changes in Pseudomonas fluorescens during naphthalene degradation.Molecular & cellular proteomics : MCP · 2013Article
- The proteasome of malaria parasites: A multi-stage drug target for chemotherapeutic intervention?International journal for parasitology. Drugs and drug resistance · 2012Review
- Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates.Journal of bacteriology · 2011Article
- Membrane proteases and aminoglycoside antibiotic resistance.Journal of bacteriology · 2011Article
- Paddling mechanism for the substrate translocation by AAA+ motor revealed by multiscale molecular simulations.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock response in Escherichia coli is autoregulated. Consistent with this, mutations in certain heat shock genes, such as dnaK, dnaJ, grpE or htrC lead to a higher constitutive heat shock gene expression at low temperatures. A similar situation occurs upon accumulation of newly synthesized peptides released prematurely from the ribosomes by puromycin. We looked for gene(s) which, when present in multicopy, prevent the constitutive heat shock response associated with htrC mutant bacteria or caused by the presence of puromycin. One such locus was identified and shown to carry the recently sequenced hslV hslU (clpQ clpY) operon. HslV/ClpQ shares a very high degree of homology with members of the beta-type subunit, constituting the catalytic core of the 20S proteasome. HslU/ClpY is 50% identical to the ClpX protein of E. coli, which is known to present large polypeptides to its partner, the ATP-independent proteolytic enzyme ClpP. We show that, in vivo, HslV and HslU interact and participate in the degradation of abnormal puromycylpolypeptides. Biochemical evidence suggests that HslV/ClpQ is an efficient peptidase whose activity is enhanced by HslU/CIpY in the presence of ATP.
Indexed as
Identifiers
9003766PMC452516W1463297302What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.