ArticleThe Biochemical journal1997
Metabolic control analysis of biochemical pathways based on a thermokinetic description of reaction rates.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Exchange of the L-cysteine exporter after in-vivo metabolic control analysis improved the L-cysteine production process with engineered Escherichia coli.Microbial cell factories · 2025Article
- Metabolic control analysis enabled the improvement of the L-cysteine production process with Escherichia coli.Applied microbiology and biotechnology · 2024Article
- Proteome allocations change linearly with the specific growth rate of Saccharomyces cerevisiae under glucose limitation.Nature communications · 2022Article
- Metabolic control analysis of L-tryptophan producing Escherichia coli applying targeted perturbation with shikimate.Bioprocess and biosystems engineering · 2021Article
- Quantification of Microbial Phenotypes.Metabolites · 2016Review
- Dynamics of benzoate metabolism inMetabolic engineering communications · 2016Article
- Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus.Journal of Zhejiang University. Science. B · 2014Article
- Pathway thermodynamics highlights kinetic obstacles in central metabolism.PLoS computational biology · 2014Article
- Development of quantitative metabolomics for Pichia pastoris.Metabolomics : Official journal of the Metabolomic Society · 2012Article
- Thermodynamic calculations for biochemical transport and reaction processes in metabolic networks.Biophysical journal · 2010Article
- Production of L-carnitine by secondary metabolism of bacteria.Microbial cell factories · 2007Article
- Thermodynamics-based metabolic flux analysis.Biophysical journal · 2007Article
- Putative regulatory sites unraveled by network-embedded thermodynamic analysis of metabolome data.Molecular systems biology · 2006Article
- Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.The Biochemical journal · 1997Article
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1 author.
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Abstract
Metabolic control analysis is a powerful technique for the evaluation of flux control within biochemical pathways. Its foundation is the elasticity coefficients and the flux control coefficients (FCCs). On the basis of a thermokinetic description of reaction rates it is here shown that the elasticity coefficients can be calculated directly from the pool levels of metabolites at steady state. The only requirement is that one thermodynamic parameter be known, namely the reaction affinity at the intercept of the tangent in the inflection point of the curve of reaction rate against reaction affinity. This parameter can often be determined from experiments in vitro. The methodology is applicable only to the analysis of simple two-step pathways, but in many cases larger pathways can be lumped into two overall conversions. In cases where this cannot be done it is necessary to apply an extension of the thermokinetic description of reaction rates to include the influence of effectors. Here the reaction rate is written as a linear function of the logarithm of the metabolite concentrations. With this type of rate function it is shown that the approach of Delgado and Liao [Biochem. J. (1992) 282, 919-927] can be much more widely applied, although it was originally based on linearized kinetics. The methodology of determining elasticity coefficients directly from pool levels is illustrated with an analysis of the first two steps of the biosynthetic pathway of penicillin. The results compare well with previous findings based on a kinetic analysis.
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