ArticleThe Biochemical journal1997
pH-dependent processing of secretogranin II by the endopeptidase PC2 in isolated immature secretory granules.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Structural Conformation and Activity of Spider-Derived Inhibitory Cystine Knot Peptide Pn3a Are Modulated by pH.ACS omega · 2023Article
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting.Life science alliance · 2022Article
- Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening.Angewandte Chemie (International ed. in English) · 2022Article
- Systematic Comparison of Vesicular Targeting Signals Leads to the Development of Genetically Encoded Vesicular Fluorescent ZnACS sensors · 2020Article
- Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production.JCI insight · 2020Article
- Simultaneous Quantification of Vesicle Size and Catecholamine Content by Resistive Pulses in Nanopores and Vesicle Impact Electrochemical Cytometry.Journal of the American Chemical Society · 2020Article
- A molecular switch orchestrates enzyme specificity and secretory granule morphology.Nature communications · 2018Article
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- Article
- Roles of myosin Va and Rab3D in membrane remodeling of immature secretory granules.Cellular and molecular neurobiology · 2010Article
- Aquaporin 1 is important for maintaining secretory granule biogenesis in endocrine cells.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Sorting of the neuroendocrine secretory protein Secretogranin II into the regulated secretory pathway: role of N- and C-terminal alpha-helical domains.The Journal of biological chemistry · 2008Article
- Discovery and progress in our understanding of the regulated secretory pathway in neuroendocrine cells.Histochemistry and cell biology · 2008Review
- Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells.The Journal of cell biology · 2006Article
- GGA function is required for maturation of neuroendocrine secretory granules.The EMBO journal · 2006Article
- Intracellular localization of the HCS2 gene products in identified snail neurons in vivo and in vitro.Cellular and molecular neurobiology · 2006Article
- Homotypic fusion of immature secretory granules during maturation requires syntaxin 6.Molecular biology of the cell · 2001Article
- Measurement of secretory vesicle pH reveals intravesicular alkalinization by vesicular monoamine transporter type 2 resulting in inhibition of prohormone cleavage.The Journal of physiology · 2001Article
- Proinsulin endoproteolysis confers enhanced targeting of processed insulin to the regulated secretory pathway.Molecular biology of the cell · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously characterized the processing of secretogranin II (SgII) in PC12 cells that were stably transfected with the endopeptidase PC2. Here we show that processing of SgII can be observed in isolated immature secretory granules (ISGs) derived from this cell line in a temperature- and ATP-dependent manner. The stimulatory effect of ATP on processing can be attributed to the activation of the vacuolar H(+)-ATPase and a concomitant decrease in intragranular pH. The immature secretory granule therefore provides an adequate environment for correct processing of SgII by PC2. The rate of SgII processing was strongly dependent on the intragranular pH, suggesting that processing of SgII can be used as a pH indicator for the granule interior. A standard curve was prepared using SgII processing in ISGs equilibrated at a range of pH values. The extent of processing in ISGs incubated in the presence of ATP at physiological pH was compared with the standard curve, and the intragranular pH was determined. From these observations, we propose an intragranular pH of 6.3 +/- 0.1 for ISGs in a physiological buffer in the presence of ATP. Hence, the pH of ISGs seems to be similar to the pH of the trans-Golgi network (TGN) and is clearly higher than the pH of mature secretory granules (pH 5.0-5.5). Interestingly, no processing of SgII could be observed in a membrane fraction that is highly enriched in TGN under conditions for which processing was readily obtained in isolated ISGs.
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