ArticleMolecular and cellular biology1997
Severe growth defect in a Schizosaccharomyces pombe mutant defective in intron lariat degradation.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 70 citations in OpenAlex.
- Intron retention in health and amyotrophic lateral sclerosis.Brain : a journal of neurology · 2026Review
- The human DBR1 interactome reveals coupling between intron lariat turnover, pre-mRNA splicing, and RNA quality control pathways.Cell stress & chaperones · 2026Article
- DBR1 orchestrates the fate of lariat RNA: debranching-dependent turnover and function.Nucleic acids research · 2025Review
- Review
- Sequestration of DBR1 to stress granules promotes lariat intronic RNAs accumulation for heat-stress tolerance.Nature communications · 2024Article
- A repertoire of intronic lariat RNAs reveals tissue-specific regulation and target mimicry potential in plants.Science China. Life sciences · 2024Article
- The debranching enzyme Dbr1 regulates lariat turnover and intron splicing.Nature communications · 2024Article
- A founder DBR1 variant causes a lethal form of congenital ichthyosis.Human genetics · 2023Article
- A functional link between lariat debranching enzyme and the intron-binding complex is defective in non-photosensitive trichothiodystrophy.Molecular cell · 2023Article
- Intron turnover is essential to the development and pathogenicity of the plant pathogenic fungus Fusarium graminearum.Communications biology · 2022Article
- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Herpes simplex virus encephalitis of childhood: inborn errors of central nervous system cell-intrinsic immunity.Human genetics · 2020Review
- A Comprehensive Map of Intron Branchpoints and Lariat RNAs in Plants.The Plant cell · 2019Article
- ShapeShifter: a novel approach for identifying and quantifying stable lariat intronic species in RNAseq data.Quantitative biology (Beijing, China) · 2018Article
- Article
- Unexplored Molecular Features of theFrontiers in cellular and infection microbiology · 2018Article
- Article
- Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic ZnFEBS letters · 2017Article
- Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The cDNAs and genes encoding the intron lariat-debranching enzyme were isolated from the nematode Caenorhabditis elegans and the fission yeast Schizosaccharomyces pombe based on their homology with the Saccharomyces cerevisiae gene. The cDNAs were shown to be functional in an interspecific complementation experiment; they can complement an S. cerevisiae dbr1 null mutant. About 2.5% of budding yeast S. cerevisiae genes have introns, and the accumulation of excised introns in a dbr1 null mutant has little effect on cell growth. In contrast, many S. pombe genes contain introns, and often multiple introns per gene, so that S. pombe is estimated to contain approximately 40 times as many introns as S. cerevisiae. The S. pombe dbr1 gene was disrupted and shown to be nonessential. Like the S. cerevisiae mutant, the S. pombe null mutant accumulated introns to high levels, indicating that intron lariat debranching represents a rate-limiting step in intron degradation in both species. Unlike the S. cerevisiae mutant, the S. pombe dbr1::leu1+ mutant had a severe growth defect and exhibited an aberrant elongated cell shape in addition to an intron accumulation phenotype. The growth defect of the S. pombe dbr1::leu1+ strain suggests that debranching activity is critical for efficient intron RNA degradation and that blocking this pathway interferes with cell growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.