ArticleMolecular and cellular biology1997
The RAD7, RAD16, and RAD23 genes of Saccharomyces cerevisiae: requirement for transcription-independent nucleotide excision repair in vitro and interactions between the gene products.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 81 citations in OpenAlex.
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- Regulation of UV damage repair in quiescent yeast cells.DNA repair · 2020Article
- The potential of the newly isolated thermotolerant3 Biotech · 2018Article
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- Mutations at the Subunit Interface of Yeast Proliferating Cell Nuclear Antigen Reveal a Versatile Regulatory Domain.PloS one · 2016Article
- Master molecule, heal thyself.The Journal of biological chemistry · 2014Article
- Nucleotide excision repair in cellular chromatin: studies with yeast from nucleotide to gene to genome.International journal of molecular sciences · 2012Review
- ABF1-binding sites promote efficient global genome nucleotide excision repair.The Journal of biological chemistry · 2009Article
- Complementary roles of yeast Rad4p and Rad34p in nucleotide excision repair of active and inactive rRNA gene chromatin.Molecular and cellular biology · 2008Article
- Mms19 protein functions in nucleotide excision repair by sustaining an adequate cellular concentration of the TFIIH component Rad3.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes.Current genetics · 2008Review
- Tfb5 interacts with Tfb2 and facilitates nucleotide excision repair in yeast.Nucleic acids research · 2007Article
- Distinct functions of the ubiquitin-proteasome pathway influence nucleotide excision repair.The EMBO journal · 2006Article
- The NEF4 complex regulates Rad4 levels and utilizes Snf2/Swi2-related ATPase activity for nucleotide excision repair.Molecular and cellular biology · 2004Article
- Rad23 stabilizes Rad4 from degradation by the Ub/proteasome pathway.Nucleic acids research · 2004Article
- Roles of Rad23 protein in yeast nucleotide excision repair.Nucleic acids research · 2004Article
- Proteolysis of a nucleotide excision repair protein by the 26 S proteasome.Current genetics · 2002Article
- Defective nucleotide excision repair in yeast hpr1 and tho2 mutants.Nucleic acids research · 2002Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Nucleotide excision repair (NER) is a biochemical process required for the repair of many different types of DNA lesions. In the yeast Saccharomyces cerevisiae, the RAD7, RAD16, and RAD23 genes have been specifically implicated in NER of certain transcriptionally repressed loci and in the nontranscribed strand of transcriptionally active genes. We have used a cell-free system to study the roles of the Rad7, Rad16, and Rad23 proteins in NER. Transcription-independent NER of a plasmid substrate was defective in rad7, rad16, and rad23 mutant extracts. Complementation studies with a previously purified NER protein complex (nucleotide excision repairosome) indicate that Rad23 is a component of the repairosome, whereas Rad7 and Rad16 proteins were not found in this complex. Complementation studies with rad4, rad7, rad16, and rad23 mutant extracts suggest physical interactions among these proteins. This conclusion was confirmed by experiments using the yeast two-hybrid assay, which demonstrated the following pairwise interactions: Rad4 with Rad23, Rad4 with Rad7, and Rad7 with Rad16. Additionally, interaction between the Rad7 and Rad16 proteins was demonstrated in vitro. Our results show that Rad7, Rad16, and Rad23 are required for transcription-independent NER in vitro. This process may involve a unique protein complex which is distinct from the repairosome and which contains at least the Rad4, Rad7, and Rad16 proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.