ArticleMolecular and cellular biology1997
CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 172 citations in OpenAlex.
- Therapeutic potential of PRMT1 as a critical survival dependency target in multiple myeloma.BMC cancer · 2025Article
- OptimizingmSphere · 2025Article
- Elevated CDC45 Expression Predicts Poorer Overall Survival Prognoses and Worse Immune Responses for Kidney Renal Clear Cell Carcinoma via Single-Cell and Bulk RNA-Sequencing.Biochemical genetics · 2024Article
- Transcriptomic Networks Reveal the Tissue-Specific Cold Shock Responses in Japanese Flounder (Biology · 2023Article
- Identification of deleterious recessive haplotypes and candidate deleterious recessive mutations in Japanese Black cattle.Scientific reports · 2021Article
- Article
- The New Paradigm of Network Medicine to Analyze Breast Cancer Phenotypes.International journal of molecular sciences · 2020Article
- Mepiquat chloride promotes cotton lateral root formation by modulating plant hormone homeostasis.BMC plant biology · 2019Article
- In the line-up: deleted genes associated with DiGeorge/22q11.2 deletion syndrome: are they all suspects?Journal of neurodevelopmental disorders · 2019Review
- The Inherent Asymmetry of DNA Replication.Annual review of cell and developmental biology · 2017Review
- Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.Current biology : CB · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Mutations in CDC45, Encoding an Essential Component of the Pre-initiation Complex, Cause Meier-Gorlin Syndrome and Craniosynostosis.American journal of human genetics · 2016Article
- Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structure of human Cdc45 and implications for CMG helicase function.Nature communications · 2016Article
- Extensive RPA2 hyperphosphorylation promotes apoptosis in response to DNA replication stress in CHK1 inhibited cells.Nucleic acids research · 2015Article
- Global Identification of Genes Specific for Rice Meiosis.PloS one · 2015Article
- Article
- The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.EMBO reports · 2014Article
- Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication.The EMBO journal · 2014Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The CDC45 gene of Saccharomyces cerevisiae was isolated by complementation of the cold-sensitive cdc45-1 mutant and shown to be essential for cell viability. Although CDC45 genetically interacts with a group of MCM genes (CDC46, CDC47, and CDC54), the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member. Further genetic characterization of the cdc45-1 mutant demonstrated that it is synthetically lethal with orc2-1, mcm2-1, and mcm3-1. These results not only reveal a functional connection between the origin recognition complex (ORC) and Cdc45p but also extend the CDC45-MCM genetic interaction to all known MCM family members that were shown to be involved in replication initiation. Initiation of DNA replication in cdc45-1 cells was defective, causing a delayed entry into S phase at the nonpermissive temperature, as well as a high plasmid loss rate which could be suppressed by tandem copies of replication origins. Furthermore, two-dimensional gels directly showed that chromosomal origins fired less frequently in cdc45-1 cells at the nonpermissive temperature. These findings suggest that Cdc45p, ORC, and Mcm proteins act in concert for replication initiation throughout the genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.