ArticleThe Journal of experimental medicine1997
Downregulation of CD1 marks acquisition of functional maturation of human thymocytes and defines a control point in late stages of human T cell development.
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- A human NK cell progenitor that originates in the thymus and generates KIRScience advances · 2025Article
- CD1b glycoprotein, a crucial marker of thymocyte development during T cell maturation in cynomolgus monkeys.Scientific reports · 2023Article
- Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells.Nature biomedical engineering · 2022Article
- Article
- Generation of highly proliferative, rejuvenated cytotoxic T cell clones through pluripotency reprogramming for adoptive immunotherapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets.Frontiers in immunology · 2019Article
- Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids.Nature methods · 2017Article
- Expression patterns of Ikaros family members during positive selection and lineage commitment of human thymocytes.Immunology · 2016Article
- Sphingosine-1-phosphate/sphingosine-1-phosphate receptor 1 signaling is required for migration of naive human T cells from the thymus to the periphery.The Journal of allergy and clinical immunology · 2016Article
- In vitro generation of mature, naive antigen-specific CD8(+) T cells with a single T-cell receptor by agonist selection.Leukemia · 2014Article
- Article
- Normal Thymic Size and Low Rate of Infections in Human Donor Milk Fed HIV-Exposed Uninfected Infants from Birth to 18 Months of Age.International journal of pediatrics · 2013Article
- Interplay between carbohydrate and lipid in recognition of glycolipid antigens by natural killer T cells.Annals of the New York Academy of Sciences · 2012Review
- Developmental regulation of P-glycoprotein activity within thymocytes results in increased anti-HIV protease inhibitor activity.Journal of leukocyte biology · 2011Article
- Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148.The Journal of biological chemistry · 2011Article
- A human thymic epithelial cell culture system for the promotion of lymphopoiesis from hematopoietic stem cells.Experimental hematology · 2011Article
- Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature.BMC immunology · 2011Article
- Ectopic cervical thymoma: a report of two cases of a rare entity frequently misdiagnosed on fine needle aspiration cytology and frozen section.Head and neck pathology · 2010Article
- Isolation and in vitro generation of gene-manipulated human plasmacytoid and conventional dendritic cells.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Restoration of adenosine deaminase-deficient human thymocyte development in vitro by inhibition of deoxynucleoside kinases.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated whether in the human thymus transition of CD4+CD8+ double positive (DP) to CD4+ or CD8+ single positive (SP) cells is sufficient for generation of functional immunocompetent T cells. Using the capacity of thymocytes to expand in vitro in response to PHA and IL-2 as a criterion for functional maturity, we found that functional maturity of both SP and DP thymocytes correlates with downregulation of CD1a. CD1a- cells with a persistent DP phenotype were also found in neonatal cord blood, suggesting that at least a proportion of mature DP cells can emigrate from the thymus. The requirements for generating functional T cells were investigated in a hybrid human/mouse fetal thymic organ culture. MHC class II-positive, but not MHC class II-negative, mouse thymic microenvironments support differentiation of human progenitors into TCR alpha beta+CD4+ SP cells, indicating that mouse MHC class II can positively select TCR alpha beta +CD4+ SP human cells. Strikingly, these SP are arrested in the CD1a+ stage and could not be expanded in vitro with PHA and IL-2. CD1a+CD4+ SP thymocytes do not represent an end stage population because purified CD1a+CD4+ SP thymocytes differentiate to expandable CD1a- cells upon cocultivation with human thymic stromal cells. Taken together these data indicate that when CD1a+ DP TCR alpha beta low cells mature, these cells interact with MHC, but that an additional, apparently species-specific, signal is required for downregulation of CD1a to generate functional mature TCR alpha beta + cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.