Evidence map›Paper›PMID 8995708›Full record

ArticleJournal of virology1997

Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes.

P B Dallas, P Yaciuk, E Moran

Open access · bronzeAbstract read
In one paragraph

Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
10.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 105 citations in OpenAlex.

  1. Muscle-specific pyruvate kinase isoforms, PKM1 and PKM2, regulate mammalian SWI/SNF proteins and histone 3 phosphorylation during myoblast differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Temporal regulation of chromatin during myoblast differentiation.Seminars in cell & developmental biology · 2017
    Review
  7. Article
  8. Article
  9. Article
  10. Dynamic acetylation of all lysine-4 trimethylated histone H3 is evolutionarily conserved and mediated by p300/CBP.Proceedings of the National Academy of Sciences of the United States of America · 2011
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

P B DallasFels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
P Yaciuk
E Moran
Temple University · US

Funding

PROTEIN INTERACTIONS AND REPRESSION IN FUNCTION OF THE ER01CA053592 · NCI · TEMPLE UNIVERSITY · PI MORAN, ELIZABETH · 1991 to 2000
$332k
CELL GROWTH CONTROL FUNCTIONS OF THE E1A ONCOGENER01CA055330 · NCI · TEMPLE UNIVERSITY · PI MORAN, ELIZABETH · 1991 to 1994
–
P64 AND P59 IN CELLULAR PROTEIN COMPLEXESR29CA068066 · NCI · SAINT LOUIS UNIVERSITY · PI YACIUK, PETER · 1995 to 1999
–
NCI NIH HHS CA53592NCI NIH HHS CA55330NCI NIH HHS CA68066
6 · The paper itself

Abstract

The amino terminus of the adenovirus E1A protein is involved in E1A transforming functions, repression of tissue-specific gene expression, and E1A-mediated enhancer repression. These N-terminal functions are associated with the ability of this region of E1A to bind to p300 and CBP, two closely related cellular proteins thought to function as transcriptional adaptor molecules. Here we describe the characterization of a panel of 11 monoclonal antibodies raised against E1A-affinity-purified 300-kDa proteins. The panel can be divided into two groups based on immunoprecipitation patterns. The first group consists of five p300/CBP-cross-reactive and two p300-specific monoclonal antibodies, all of which immunoprecipitate p300 and/or CBP without associated cellular proteins. In contrast, the second group immunoprecipitates p300 or both p300 and CBP in association with a complex of at least seven other cellular proteins. Taking advantage of the specificities of these monoclonal antibodies, we have identified both p300 and CBP in in vivo complexes with TBP, a finding consistent with a role for both p300 and CBP in promoting interactions between upstream promoter elements and the basal transcription apparatus.

Indexed as

Trans-ActivatorsAdenoviridaeAdenovirus E1A ProteinsAmino Acid SequenceAntibodies, MonoclonalAntibodies, ViralCREB-Binding ProteinDNA-Binding ProteinsEpitope MappingMolecular Sequence DataNuclear ProteinsPhosphoproteinsTATA-Box Binding ProteinTranscription FactorsAdenovirus E1A ProteinsAntibodies, MonoclonalAntibodies, ViralCREB-Binding ProteinDNA-Binding ProteinsNuclear ProteinsPhosphoproteinsTATA-Box Binding ProteinTrans-ActivatorsTranscription Factors

Identifiers

PMID8995708
PMCPMC191239
OpenAlexW2163074889

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.