ArticleJournal of virology1997
Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Interaction with TopBP1 Is Required for Human Papillomavirus 16 E2 Plasmid Segregation/Retention Function during Mitosis.Journal of virology · 2022Article
- Article
- Using a Human Papillomavirus Model to Study DNA Replication and Repair of Wild Type and Damaged DNA Templates in Mammalian Cells.International journal of molecular sciences · 2020Review
- Article
- Activating the DNA Damage Response and Suppressing Innate Immunity: Human Papillomaviruses Walk the Line.Pathogens (Basel, Switzerland) · 2020Review
- Correlation of HPV16 Gene Status and Gene Expression With Antibody Seropositivity and TIL Status in OPSCC.Frontiers in oncology · 2020Article
- Article
- Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16.BMC cancer · 2017Article
- Characterization of the Human Papillomavirus 16 E8 Promoter.Journal of virology · 2015Article
- Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication.Journal of virology · 2015Article
- A conserved regulatory module at the C terminus of the papillomavirus E1 helicase domain controls E1 helicase assembly.Journal of virology · 2015Article
- Regulation of human genome expression and RNA splicing by human papillomavirus 16 E2 protein.Virology · 2014Article
- Replication interference between human papillomavirus types 16 and 18 mediated by heterologous E1 helicases.Virology journal · 2014Article
- The E1 proteins.Virology · 2013Review
- The papillomavirus E2 proteins.Virology · 2013Review
- Gene- and protein-delivered zinc finger-staphylococcal nuclease hybrid for inhibition of DNA replication of human papillomavirus.PloS one · 2013Article
- Article
- Characterization and whole genome analysis of human papillomavirus type 16 e1-1374^63nt variants.PloS one · 2012Article
- A conserved amphipathic helix in the N-terminal regulatory region of the papillomavirus E1 helicase is required for efficient viral DNA replication.Journal of virology · 2011Article
- Human papillomavirus type 18 DNA load and 2-year cumulative diagnoses of cervical intraepithelial neoplasia grades 2-3.Journal of the National Cancer Institute · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The papillomavirus E1 and E2 proteins are both necessary and sufficient in vivo for efficient origin-dependent viral DNA replication. The ability of E1 and E2 to complex with each other appears to be essential for efficient viral DNA replication. In this study, we used the yeast two-hybrid system and in vitro binding assays to map the domains of the human papillomavirus type 16 (HPV16) E1 and E2 proteins required for complex formation. The amino-terminal 190-amino-acid domain of HPV16 E2 was both required and sufficient for E1 binding. The carboxyl-terminal 229 amino acids of E 1 were essential for binding E2, and the amino-terminal 143 amino acids of HPV16 E1 were dispensable. Although the ability of the E1 minimal domain (amino acids [aa] 421 to 649) to interact with E2 was strong at 4 degrees C, it was significantly reduced at temperatures above 25 degrees C. A larger domain of E1 from aa 144 to 649 bound E2 efficiently at any temperature, suggesting that aa 144 to 420 of E1 may play a role in the HPV16 E1-E2 interaction at physiological temperatures.
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