ArticleThorax1996
Binding of Aspergillus fumigatus spores to lung epithelial cells and basement membrane proteins: relevance to the asthmatic lung.
Article in Thorax, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2023Review
- Protective role of CFTR during fungal infection of cystic fibrosis bronchial epithelial cells withFrontiers in cellular and infection microbiology · 2023Article
- Review
- Basement membranes in obstructive pulmonary diseases.Matrix biology plus · 2021Review
- Mechanisms of fungal dissemination.Cellular and molecular life sciences : CMLS · 2021Review
- Phagolysosomal Survival Enables Non-lytic Hyphal Escape and Ramification Through Lung Epithelium DuringFrontiers in microbiology · 2020Article
- How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.Journal of molecular biology · 2019Review
- It takes a village: Phagocytes play a central role in fungal immunity.Seminars in cell & developmental biology · 2019Review
- Lung colonization by Aspergillus fumigatus is controlled by ZNF77.Nature communications · 2018Article
- Aspergillus fumigatus adhesion factors in dormant conidia revealed through comparative phenotypic and transcriptomic analyses.Cellular microbiology · 2018Article
- Anti-Aspergillus Activities of the Respiratory Epithelium in Health and Disease.Journal of fungi (Basel, Switzerland) · 2018Review
- FleA Expression in Aspergillus fumigatus Is Recognized by Fucosylated Structures on Mucins and Macrophages to Prevent Lung Infection.PLoS pathogens · 2016Article
- Interactions of Aspergillus fumigatus Conidia with Airway Epithelial Cells: A Critical Review.Frontiers in microbiology · 2016Review
- Host cell invasion by medically important fungi.Cold Spring Harbor perspectives in medicine · 2014Review
- Article
- Identification of fibrinogen-binding proteins of Aspergillus fumigatus using proteomic approach.Mycopathologia · 2012Article
- Interaction of the pathogenic mold Aspergillus fumigatus with lung epithelial cells.Frontiers in microbiology · 2012Article
- Development of a novel ex vivo model of corneal fungal adherence.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2011Article
- Allergic bronchopulmonary aspergillosis in asthma and cystic fibrosis.Clinical & developmental immunology · 2011Review
- Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis.Clinical microbiology reviews · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAspergillus fumigatus is an opportunistic pathogen to which asthmatic subjects are particularly susceptible. The ability of spores of A fumigatus to bind to pulmonary cells and basement membrane proteins was investigated to determine the mechanisms involved in this susceptibility.
methodsCells of the A549 pulmonary epithelial cell line or purified basement membrane proteins were immobilised on the wells of microtitre plates. They were then exposed to spores of A fumigatus in suspension, with or without various pretreatments of the spores, cells, and proteins. Adherent spores were counted by light microscopy.
resultsSpores of A fumigatus bound in a concentration dependent manner to A549 epithelial cells and pretreatment of cells with interferon gamma (2500 units/ml) caused a significant doubling of spore binding. Binding of spores to A549 cells was inhibited by about a third by pre-incubation of the spores with fibrinogen (100 micrograms/ml). Spores bound specifically to extracellular matrix (ECM) components laid down by A549 cells, and pretreatment of the ECM components with hydrogen peroxide (25-80 microM) enhanced spore binding by approximately one third. They also bound specifically and in a saturable manner to purified fibrinogen, fibronectin, laminin, type I collagen, and type IV collagen. Pre-incubation of spores with Arg-Gly-Asp tripeptide (RGD; 50-200 micrograms/ ml) inhibited binding to fibronectin and type I collagen by 50%.
conclusionsThis study suggests that the presence of activated epithelial cells and the exposure of basement membrane that occurs in asthma, together with oxidant stress, may facilitate the colonisation of the asthmatic lung by A fumigatus. The RGD sequence may be involved in spore binding to some ECM proteins. Free fibrinogen may protect against binding of A fumigatus spores to the pulmonary epithelium.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.