ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Overexpression of Ca2+/calmodulin-dependent protein kinase II in PC12 cells alters cell growth, morphology, and nerve growth factor-induced differentiation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Protein kinases: master regulators of neuritogenesis and therapeutic targets for axon regeneration.Cellular and molecular life sciences : CMLS · 2020Review
- Chronic ethanol increases calcium/calmodulin-dependent protein kinaseIIδ gene expression and decreases monoamine oxidase amount in rat heart muscles: Rescue effect of Zingiber officinale (ginger) extract.Anatolian journal of cardiology · 2018Article
- Prolyl Isomerase Pin1 Directly Regulates Calcium/Calmodulin-Dependent Protein Kinase II Activity in Mouse Brains.Frontiers in pharmacology · 2018Article
- Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation.Journal of radiation research · 2017Article
- C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity.Molecular biology of the cell · 2017Article
- Article
- Conditional deletion of α-CaMKII impairs integration of adult-generated granule cells into dentate gyrus circuits and hippocampus-dependent learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Proarrhythmic defects in Timothy syndrome require calmodulin kinase II.Circulation · 2008Article
- Regulation of exocytosis through Ca2+/ATP-dependent binding of autophosphorylated Ca2+/calmodulin-activated protein kinase II to syntaxin 1A.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Multiple distinct signal pathways, including an autocrine neurotrophic mechanism, contribute to the survival-promoting effect of depolarization on spiral ganglion neurons in vitro.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Receptor subtype-induced targeting and subtype-specific internalization of human alpha(2)-adrenoceptors in PC12 cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Laminin directs growth cone navigation via two temporally and functionally distinct calcium signals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
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Authors and funding
2 authors.
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Abstract
To examine the role of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in cell differentiation and neuronal functions, stable transformants of PC12 cells were established that expressed levels of the alpha-subunit of CaMKII (alpha CaMKII) equivalent to mammalian neurons. The expression of the transfected alpha CaMKII gene or the endogenous beta CaMKII gene was monitored by RNase protection assays, and alpha CaMKII protein expression was determined by Western blots. Several PC12-derived clones expressed amounts of alpha CaMKII mRNA and alpha CaMKII protein similar to that of hippocampal tissues and several orders of magnitude greater than untransfected PC12 cells. CaMKII catalytic activity was four times higher in extracts from alpha CaMKII-overexpressing compared with untransfected PC12 cells. All clones overexpressing alpha CaMKII displayed altered cellular growth and adhesion properties including increased cell-to-substrate adhesion, decreased cell-to-cell adhesion, enhanced contact inhibition, and prolonged survival at confluency. Furthermore, the alpha CaMKII activity in overexpressing PC12 cells inhibited neurite elongation during NGF-induced differentiation. Inhibition of CaMKII activity in vivo with KN-62 caused the morphological phenotypes of alpha CaMKII-overexpressing cells to partially revert to that of untransfected PC12 cells. These results show that alpha CaMKII catalytic activity affects growth, morphology, and NGF-induced differentiation of PC12 cells.
Indexed as
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8994047PMC6573171What OpenQuestion holds
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