ReviewClinical microbiology reviews1997
PCR in laboratory diagnosis of human Borrelia burgdorferi infections.
Review in Clinical microbiology reviews, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 169 citations in OpenAlex.
- Histological staining of tick bite skin biopsies for spirochetes and Powassan virus RNA.Microbiology spectrum · 2025Article
- Tailored Functionalization of Plasmonic AgNPs/C:H:N:O Nanocomposite for Sensitive and Selective Detection.Journal of biophotonics · 2025Article
- Development, Optimization, and Validation of a Quantitative PCR Assay forPathogens (Basel, Switzerland) · 2024Article
- Large-Scale Sequencing ofGenes · 2022Review
- Molecular Detection of Tick-Borne Pathogens in American Bison (Pathogens (Basel, Switzerland) · 2021Article
- Article
- Article
- Lyme Disease Frontiers: ReconcilingPathogens (Basel, Switzerland) · 2019Review
- Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR.Journal of visualized experiments : JoVE · 2018Article
- History of Morgellons disease: from delusion to definition.Clinical, cosmetic and investigational dermatology · 2018Review
- Article
- Morgellons disease: a filamentous borrelial dermatitis.International journal of general medicine · 2016Review
- Detection, identification and genotyping of Borrellia spp. in rodents in Slovenia by PCR and culture.BMC veterinary research · 2015Article
- Exploring the association between Morgellons disease and Lyme disease: identification of Borrelia burgdorferi in Morgellons disease patients.BMC dermatology · 2015Article
- Culture and identification ofF1000Research · 2014Article
- Application of medical and analytical methods in Lyme borreliosis monitoring.Analytical and bioanalytical chemistry · 2012Review
- Detection of Borrelia persica Infection in Ornithodoros tholozani Using PCR Targeting rrs Gene and Xenodiagnosis.Iranian journal of public health · 2011Article
- Novel Diagnosis of Lyme Disease: Potential for CAM Intervention.Evidence-based complementary and alternative medicine : eCAM · 2009Article
- Validation of cultivation and PCR methods for diagnosis of Lyme neuroborreliosis.Journal of clinical microbiology · 2008Article
- Emerging pathogens: challenges and successes of molecular diagnostics.The Journal of molecular diagnostics : JMD · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The laboratory diagnosis of Lyme borreliosis, the most prevalent vector-borne disease in the United States and endemic in parts of Europe and Asia, is currently based on serology with known limitations. Direct demonstration of Borrelia burgdorferi by culture may require weeks, while enzyme-linked immunosorbent assays for antigen detection often lack sensitivity. The development of the PCR has offered a new dimension in the diagnosis. Capable of amplifying minute amounts of DNA into billions of copies in just a few hours, PCR facilitates the sensitive and specific detection of DNA or RNA of pathogenic organisms. This review is restricted to applications of PCR methods in the diagnosis of human B. burgdorferi infections. In the first section, methodological aspects, e.g., sample preparation, target selection, primers and PCR methods, and detection and control of inhibition and contamination, are highlighted. In the second part, emphasis is placed on diagnostic aspects, where PCR results in patients with dermatological, neurological, joint, and ocular manifestations of the disease are discussed. Here, special attention is given to monitoring treatment efficacy by PCR tests. Last, specific guidelines on how to interpret PCR results, together with the advantages and limitations of these new techniques, are presented.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.