ArticleThe Journal of cell biology1996
Targeting of Moloney murine leukemia virus gag precursor to the site of virus budding.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding.Retrovirology · 2016Article
- Murine leukemia virus Gag localizes to the uropod of migrating primary lymphocytes.Journal of virology · 2014Article
- Foamy virus budding and release.Viruses · 2013Review
- Retroviral matrix and lipids, the intimate interaction.Retrovirology · 2011Review
- A replication-incompetent Rift Valley fever vaccine: chimeric virus-like particles protect mice and rats against lethal challenge.Virology · 2010Article
- Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrix.Journal of virology · 2010Article
- Purification of infectious human herpesvirus 6A virions and association of host cell proteins.Virology journal · 2007Article
- Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production.Virology journal · 2006Article
- Vpu and Tsg101 regulate intracellular targeting of the human immunodeficiency virus type 1 core protein precursor Pr55gag.Journal of virology · 2006Article
- Intracellular assembly and budding of the Murine Leukemia Virus in infected cells.Retrovirology · 2006Article
- Passive and active inclusion of host proteins in human immunodeficiency virus type 1 gag particles during budding at the plasma membrane.Journal of virology · 2004Article
- Human immunodeficiency virus type 1 assembly and lipid rafts: Pr55(gag) associates with membrane domains that are largely resistant to Brij98 but sensitive to Triton X-100.Journal of virology · 2003Article
- Interactions between the transmembrane segments of the alphavirus E1 and E2 proteins play a role in virus budding and fusion.Journal of virology · 2003Article
- Murine retroviral pseudotype virus containing hepatitis B virus large and small surface antigens confers specific tropism for primary human hepatocytes: a potential liver-specific targeting system.Journal of virology · 2002Article
- Intracellular distribution of human T-cell leukemia virus type 1 Gag proteins is independent of interaction with intracellular membranes.Journal of virology · 2002Article
- RNA is a structural element in retrovirus particles.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Kinetic analysis of human immunodeficiency virus type 1 assembly reveals the presence of sequential intermediates.Journal of virology · 2000Article
- Minimal exclusion of plasma membrane proteins during retroviral envelope formation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Human cytomegalovirus pp28 (UL99) localizes to a cytoplasmic compartment which overlaps the endoplasmic reticulum-golgi-intermediate compartment.Journal of virology · 2000Article
- Trafficking of an acylated cytosolic protein: newly synthesized p56(lck) travels to the plasma membrane via the exocytic pathway.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retrovirus Moloney murine leukemia virus (M-MuLV) matures by budding at the cell surface. Central to the budding process is the myristoylated viral core protein precursor Gag which, even in the absence of all other viral components, is capable of associating with the cytoplasmic leaflet of the plasma membrane and assembling into extracellular virus-like particles. In this paper we have used heterologous, Semliki Forest virus-driven, expression of M-MuLV Gag to study the mechanism by which this protein is targeted to the cell surface. In pulse-chase experiments, BFA, monensin, and 20 degrees C block did not affect incorporation of Gag into extracellular particles thereby indicating that the secretory pathway is not involved in targeting of Gag to the cell surface. Subcellular fractionation studies demonstrated that newly synthesized Gag became rapidly and efficiently associated with membranes which had a density similar to that of plasma membrane-derived vesicles. Protease-protection studies confirmed that the Gag-containing membranes were of plasma membrane origin, since in crude cell homogenates, the bulk of newly synthesized Gag was protease-resistant as expected of a protein that binds to the cytoplasmic leaflet of the plasma membrane. Taken together these data indicate that targeting of M-MuLV Gag to the cell surface proceeds via direct insertion of the protein to the cytoplasmic side of the plasma membrane. Furthermore, since the membrane insertion reaction is highly efficient and specific, this suggests that the reaction is dependent on as-yet-unidentified cellular factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.