ArticleThe Journal of cell biology1996
Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 225 citations in OpenAlex.
- Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivity.Scientific reports · 2021Article
- Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.Frontiers in molecular neuroscience · 2021Article
- A dual role for βII-spectrin in axons.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- βII-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal organelle transport.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Spectrin regulates cell contractility through production and maintenance of actin bundles in the Caenorhabditis elegans spermatheca.Molecular biology of the cell · 2018Article
- The cytoplasmic dynein transport machinery and its many cargoes.Nature reviews. Molecular cell biology · 2018Review
- β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity.Molecular biology of the cell · 2017Article
- Dynactin has two antagonistic regulatory domains and exerts opposing effects on dynein motility.PloS one · 2017Article
- The structure of the dynactin complex and its interaction with dynein.Science (New York, N.Y.) · 2015Article
- A PIK3C3-ankyrin-B-dynactin pathway promotes axonal growth and multiorganelle transport.The Journal of cell biology · 2014Article
- Autoregulatory mechanism for dynactin control of processive and diffusive dynein transport.Nature cell biology · 2014Article
- Review
- PKA-dependent dynein switching from lysosomes to adenovirus: a novel form of host-virus competition.The Journal of cell biology · 2014Article
- Axonal transport and neurodegenerative disease: vesicle-motor complex formation and their regulation.Degenerative neurological and neuromuscular disease · 2014Review
- Ordered recruitment of dynactin to the microtubule plus-end is required for efficient initiation of retrograde axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Fodrin in centrosomes: implication of a role of fodrin in the transport of gamma-tubulin complex in brain.PloS one · 2013Article
- BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures.Molecular biology of the cell · 2012Article
- Dynactin's pointed-end complex is a cargo-targeting module.Molecular biology of the cell · 2012Article
- Analyses of dynein heavy chain mutations reveal complex interactions between dynein motor domains and cellular dynein functions.Genetics · 2012Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Centractin (Arp1), an actin-related protein, is a component of the dynactin complex. To investigate potential functions of the protein, we used transient transfections to overexpress centractin in mammalian cells. We observed that the overexpressed polypeptide formed filamentous structures that were significantly longer and more variable in length than those observed in the native dynactin complex. The centractin filaments were distinct from conventional actin in subunit composition and pharmacology as demonstrated by the absence of immunoreactivity of these filaments with an actin-specific antibody, by resistance to treatment with the drug cytochalasin D, and by the inability to bind phalloidin. We examined the transfected cells for evidence of specific associations of the novel centractin filaments with cellular organelles or cytoskeletal proteins. Using immunocytochemistry we observed the colocalization of Golgi marker proteins with the centractin polymers. Additional immunocytochemical analysis using antibodies to non-erythroid spectrin (fodrin) and Golgi-spectrin (beta I sigma *) revealed that spectrin colocalized with the centractin filaments in transfected cells. Biochemical assays demonstrated that spectrin was present in dynactin-enriched cellular fractions, was coimmunoprecipitated from rat brain cytosol using antibodies to dynactin subunits, and was coeluted with dynactin using affinity chromatography. Immunoprecipitations and affinity chromatography also revealed that actin is not a bona fide component of dynactin. Our results indicate that spectrin is associated with the dynactin complex. We suggest a model in which dynactin associates with the Golgi through an interaction between the centractin filament of the dynactin complex and a spectrin-linked cytoskeletal network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.