ArticleProceedings of the National Academy of Sciences of the United States of America1997
In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 133 citations in OpenAlex.
- Barcoding Microtubules: Encoding Information onto Macromolecules by Photobleaching.Nano letters · 2025Article
- Plant Kinesin Repertoires Expand with New Domain Architecture and Contract with the Loss of Flagella.Journal of molecular evolution · 2024Article
- The localization of PHRAGMOPLAST ORIENTING KINESIN1 at the division site depends on the microtubule-binding proteins TANGLED1 and AUXIN-INDUCED IN ROOT CULTURES9 in Arabidopsis.The Plant cell · 2022Article
- An estimate to the first approximation of microtubule rupture force.European biophysics journal : EBJ · 2019Article
- A rice class-XIV kinesin enters the nucleus in response to cold.Scientific reports · 2018Article
- Phragmoplast microtubule dynamics - a game of zones.Journal of cell science · 2018Review
- The Cytoskeleton and Its Regulation by Calcium and Protons.Plant physiology · 2016Review
- Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.Nature plants · 2015Article
- PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana.Journal of experimental botany · 2015Article
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
- Article
- Plant Kinesin-Like Calmodulin Binding Protein Employs Its Regulatory Domain for Dimerization.PloS one · 2013Article
- The novel functions of kinesin motor proteins in plants.Protoplasma · 2012Review
- Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant.PloS one · 2012Article
- A kinesin motor in a force-producing conformation.BMC structural biology · 2010Article
- Costal2 functions as a kinesin-like protein in the hedgehog signal transduction pathway.Current biology : CB · 2008Article
- Comprehensive comparative analysis of kinesins in photosynthetic eukaryotes.BMC genomics · 2006Article
- A bidirectional kinesin motor in live Drosophila embryos.Traffic (Copenhagen, Denmark) · 2005Article
- Kar3 interaction with Cik1 alters motor structure and function.The EMBO journal · 2005Article
- Origin and evolution of Kinesin-like calmodulin-binding protein.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
AtKCBP is a calcium-dependent calmodulin-binding protein from Arabidopsis that contains a conserved kinesin microtubule motor domain. Calmodulin has been shown previously to bind to heavy chains of the unconventional myosins, where it is required for in vitro motility of brush border myosin I, but AtKCBP is the first kinesin-related heavy chain reported to be capable of binding specifically to calmodulin. Other kinesin proteins have been identified in Arabidopsis, but none of these binds to calmodulin, and none has been demonstrated to be a microtubule motor. We have tested bacterially expressed AtKCBP for the ability to bind microtubules to a glass surface and induce gliding of microtubules across the glass surface. We find that AtKCBP is a microtubule motor protein that moves on microtubules toward the minus ends, with the opposite polarity as kinesin. In the presence of calcium and calmodulin, AtKCBP no longer binds microtubules to the coverslip surface. This contrasts strikingly with the requirement of calmodulin for in vitro motility of brush border myosin I. Calmodulin could regulate AtKCBP binding to microtubules in the cell by inhibiting the binding of the motor to microtubules. The ability to bind to calmodulin provides an evolutionary link between the kinesin and myosin motor proteins, but our results indicate that the mechanisms of interaction and regulation of kinesin and myosin heavy chains by calmodulin are likely to differ significantly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.