ArticleProceedings of the National Academy of Sciences of the United States of America1997
Surface dynamics in living acinar cells imaged by atomic force microscopy: identification of plasma membrane structures involved in exocytosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 269 citations in OpenAlex.
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- Porosomes in uterine epithelial cells: Ultrastructural identification and characterization during early pregnancy.Journal of morphology · 2022Article
- Cell Surface Binding and Lipid Interactions behind Chemotherapy-Drug-Induced Ion Pore Formation in Membranes.Membranes · 2021Article
- Application of atomic force microscope in diagnosis of single cancer cells.Biomicrofluidics · 2020Review
- Nanoscale imaging using differential expansion microscopy.Histochemistry and cell biology · 2020Article
- Visualization of internal in situ cell structure by atomic force microscopy.Histochemistry and cell biology · 2018Review
- Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.Discoveries (Craiova, Romania) · 2017Review
- Human Platelet Vesicles Exhibit Distinct Size and Proteome.Journal of proteome research · 2017Article
- How do you recognize and reconstitute a synaptic vesicle after fusion?F1000Research · 2017Review
- Article
- The neuronal porosome complex in health and disease.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Functional Reconstitution of the Insulin-Secreting Porosome Complex in Live Cells.Endocrinology · 2016Article
- 'Porosome' discovered nearly 20 years ago provides molecular insights into the kiss-and-run mechanism of cell secretion.Journal of cellular and molecular medicine · 2015Review
- Neuronal porosome lipidome.Journal of cellular and molecular medicine · 2014Article
- Porosome: a membrane microdomain acting as the universal secretory portal in exocytosis.Discoveries (Craiova, Romania) · 2014Review
- Insights into the Nanobiology of Growth Hormone Secretion.Discoveries (Craiova, Romania) · 2014Article
- Discoveries Interview: Professor Bhanu P. Jena on the discovery of the porosome, the universal machinery for cellular secretion.Discoveries (Craiova, Romania) · 2014Article
- NeuronalJournal of molecular structure · 2014Article
- White noise and neuronal porosome complex: transmission electron microscopic study.Discoveries (Craiova, Romania) · 2014Article
- Porosome in the Exocrine Pancreas: A Detailed EM Study suppressor.Discoveries (Craiova, Romania) · 2014Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dynamics at the plasma membrane resulting from secretory vesicle docking and fusion and compensatory endocytosis has been difficult to observe in living cells primarily due to limited resolution at the light microscopic level. Using the atomic force microscope, we have been able to image and record changes in plasma membrane structure at ultrahigh resolution after stimulation of secretion from isolated pancreatic acinar cells. "Pits" measuring 500-2000 nm and containing 3-20 depressions measuring 100-180 nm in diameter were observed only at the apical region of acinar cells. The time course of an increase and decrease in "depression" size correlated with an increase and decrease of amylase secretion from live acinar cells. Depression dynamics and amylase release were found to be regulated in part by actin. No structural changes were identified at the basolateral region of these cells. Our results suggest depressions to be the fusion pores identified earlier in mast cells by freeze-fracture electron microscopy and by electrophysiological measurements. The atomic force microscope has enabled us to observe plasma membrane dynamics of the exocytic process in living cells in real time.
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