ArticleProceedings of the National Academy of Sciences of the United States of America1997
Mutation of gene-proximal regulatory elements disrupts human epsilon-, gamma-, and beta-globin expression in yeast artificial chromosome transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Engineering Globin Gene Expression.Molecular therapy. Methods & clinical development · 2019Review
- Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse.PloS one · 2019Article
- Genomic editing tools to model human diseases with isogenic pluripotent stem cells.Stem cells and development · 2014Review
- Shadow enhancers: frequently asked questions about distributed cis-regulatory information and enhancer redundancy.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- Article
- Silencing of Agamma-globin gene expression during adult definitive erythropoiesis mediated by GATA-1-FOG-1-Mi2 complex binding at the -566 GATA site.Molecular and cellular biology · 2008Article
- Multiple strategies for gene transfer, expression, knockdown, and chromatin influence in mammalian cell lines and transgenic animals.Molecular biotechnology · 2006Review
- Investigations of a human embryonic globin gene silencing element using YAC transgenic mice.Experimental biology and medicine (Maywood, N.J.) · 2006Article
- Initiation of DNA replication at the human beta-globin 3' enhancer.Nucleic acids research · 2005Article
- Multiple interactions between regulatory regions are required to stabilize an active chromatin hub.Genes & development · 2004Article
- Sequences in the (A)gamma-delta intergenic region are not required for stage-specific regulation of the human beta-globin gene locus.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Spatial organization of gene expression: the active chromatin hub.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Developmentally dynamic histone acetylation pattern of a tissue-specific chromatin domain.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.Genes & development · 2000Article
- The polyoma virus enhancer cannot substitute for DNase I core hypersensitive sites 2-4 in the human beta-globin LCR.Nucleic acids research · 1999Article
- Hypersensitive site 2 specifies a unique function within the human beta-globin locus control region to stimulate globin gene transcription.Molecular and cellular biology · 1999Article
- Localization of distant urogenital system-, central nervous system-, and endocardium-specific transcriptional regulatory elements in the GATA-3 locus.Molecular and cellular biology · 1999Article
- Rescue of the embryonic lethal hematopoietic defect reveals a critical role for GATA-2 in urogenital development.The EMBO journal · 1998Article
- Substitution of the human beta-spectrin promoter for the human agamma-globin promoter prevents silencing of a linked human beta-globin gene in transgenic mice.Molecular and cellular biology · 1998Article
- The A gamma-globin 3' element provides no unique function(s) for human beta-globin locus gene regulation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies have defined transcriptional control elements, in addition to the promoters, that both lie near individual human beta-globin locus genes and have been implicated in their differential stage-specific regulation during development (i.e., are believed to directly participate in hemoglobin switching). We have reinvestigated the activities during erythropoiesis that might be conferred by two of the more intensively analyzed of these elements, the epsilon-globin gene 5' silencer and the beta-globin gene 3' enhancer, by deleting them from a yeast artificial chromosome that spans the human beta-globin locus, and then analyzing transgenic mice for expression of all of the human genes. These studies show that sequences within the epsilon-globin "silencer" are not only required for silencing but are also required for activation of epsilon-globin transcription; furthermore, deletion of the silencer simultaneously reduced gamma-globin transcription during the yolk sac stage of erythroid development. Analysis of the adult beta-globin gene 3' enhancer deletion showed that its deletion affects only that gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.