ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Translocation of RNA granules in living neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 177 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
177 citing papers in PubMed.
- Synaptic Proteostasis in Health and Disease.Journal of neurochemistry · 2026Review
- Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons.The Journal of biological chemistry · 2026Article
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain.bioRxiv : the preprint server for biology · 2026Article
- Dynamic structural plasticity facilitates the developmental assembly of cochlear inner hair cell ribbon synapses.Frontiers in cell and developmental biology · 2026Article
- Dysregulated neuronal mRNA transport and translation in FTD/ALS.NPJ dementia · 2026Review
- Multiplexed smFISH Reveals the Spatial Organization of Neuropil Localized mRNAs Is Linked to Abundance.eNeuro · 2025Article
- Biomolecular condensates: molecular structure, biological functions, diseases, and therapeutic targets.Molecular biomedicine · 2025Review
- Local Protein Synthesis at Synapses: A Driver for Synapse Diversification.Journal of neurochemistry · 2025Review
- Translation stalling in neurons: a critical mechanism for timely protein delivery to distal cellular processes.Biochemical Society transactions · 2025Article
- Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development.eLife · 2025Article
- RNA-binding proteins in ALS and FTD: from pathogenic mechanisms to therapeutic insights.Molecular neurodegeneration · 2025Review
- Decoding the complex journeys of RNAs along neurons.Nucleic acids research · 2025Article
- ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.Nature protocols · 2025Review
- Decoding subcellular RNA localization one molecule at a time.Genome biology · 2025Review
- How energy determines spatial localisation and copy number of molecules in neurons.Nature communications · 2025Article
- RNA granules in flux: dynamics to balance physiology and pathology.Nature reviews. Neuroscience · 2024Review
- Cytoplasmic ribosomes hitchhike on mitochondria to dendrites.bioRxiv : the preprint server for biology · 2024Article
- Synapse-specific structural plasticity that protects and refines local circuits during LTP and LTD.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Polysome collapse and RNA condensation fluidize the cytoplasm.Molecular cell · 2024Article
- Mechanistic insights into the basis of widespread RNA localization.Nature cell biology · 2024Review
117 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Sorting of RNAs to specific subcellular loci occurs in diverse settings from fly oocytes to mammalian neurons. Using the membrane-permeable nucleic acid stain SYTO 14, we directly visualized the translocation of endogenous RNA in living cells. Labeled RNA was distributed nonrandomly as discrete granules in neuronal processes. The labeled granules colocalized with poly(A+) mRNA, with the 60S ribosomal subunit, and with elongation factor 1alpha, suggesting that granules represent a translational unit. A subset of labeled granules colocalized with beta-actin mRNA. Correlative light and electron microscopy indicated that the fluorescent granules corresponded to clusters of ribosomes at the ultrastructural level. Poststaining of sections with heavy metals confirmed the presence of ribosomes within these granules. In living neurons, a subpopulation of RNA granules was motile during the observation period. They moved at an average rate of 0.1 microm/sec. In young cultures their movements were exclusively anterograde, but after 7 d in culture, one-half of the motile granules moved in the retrograde direction. Granules in neurites were delocalized after treatment with microtubule-disrupting drugs. These results raise the possibility of a cellular trafficking system for the targeting of RNA in neurons.
Indexed as
Identifiers
8987809PMC6579227What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.