ReviewMicrobiological reviews1996
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.
Review in Microbiological reviews, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 206 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
206 citing papers in PubMed, 776 citations in OpenAlex.
- Biofilm-mediated antibiotic cross-protection:Microbiology spectrum · 2026Article
- Safety-constrained evaluation of antibacterial multicomponent borate glasses.Biomaterials and biosystems · 2026Article
- Replicative age differentially predicts metabolic competence across industrial yeasts at single-cell resolution.FEMS yeast research · 2026Article
- Rapid accumulation of fluorophores and fast kill identify drugs with bactericidal effects against Gram-negative bacteria.Microbiology (Reading, England) · 2025Article
- Monitoring the Metabolic Activity of a Single Bacterial Cell Based on Scattering Intensity.Analytical chemistry · 2025Article
- Combining single-cell ATAC and RNA sequencing for supervised cell annotation.BMC bioinformatics · 2025Article
- Development of flow cytometry assay to quantify packaging of C. jejuni by Tetrahymena species.FEMS microbiology letters · 2025Article
- Identification and monitoring of cell heterogeneity from plasmid recombination during limonene production.Applied microbiology and biotechnology · 2025Article
- Article
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.Science advances · 2024Article
- Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient.Biomicrofluidics · 2024Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- The Biology and Biochemistry of Kynurenic Acid, a Potential Nutraceutical with Multiple Biological Effects.International journal of molecular sciences · 2024Review
- Evidence for the Role of the Mitochondrial ABC Transporter MDL1 in the Uptake of Clozapine and Related Molecules into the YeastPharmaceuticals (Basel, Switzerland) · 2024Article
- Identifying antibiotic-resistant strains via cell sorting and elastic-light-scatter phenotyping.Applied microbiology and biotechnology · 2024Article
- Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.Trends in analytical chemistry : TRAC · 2024Article
- Determination of Ploidy Levels and Nuclear DNA Content inJournal of fungi (Basel, Switzerland) · 2024Article
- Ionic liquid-coated lipid nanoparticles increase siRNA uptake into CNS targets.Nanoscale advances · 2024Article
- The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase.Microbial cell factories · 2024Article
- Enhancing microbiologically influenced corrosion protection of carbon steels with silanized epoxy-biocide hybrid coatings.Environmental science and pollution research international · 2024Article
146 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most fundamental questions such as whether a cell is alive, in the sense of being able to divide or to form a colony, may sometimes be very hard to answer, since even axenic microbial cultures are extremely heterogeneous. Analyses that seek to correlate such things as viability, which is a property of an individual cell, with macroscopic measurements of culture variables such as ATP content, respiratory activity, and so on, must inevitably fail. It is therefore necessary to make physiological measurements on individual cells. Flow cytometry is such a technique, which allows one to analyze cells rapidly and individually and permits the quantitative analysis of microbial heterogeneity. It therefore offers many advantages over conventional measurements for both routine and more exploratory analyses of microbial properties. While the technique has been widely applied to the study of mammalian cells, is use in microbiology has until recently been much more limited, largely because of the smaller size of microbes and the consequently smaller optical signals obtainable from them. Since these technical barriers no longer hold, flow cytometry with appropriate stains has been used for the rapid discrimination and identification of microbial cells, for the rapid assessment of viability and of the heterogeneous distributions of a wealth of other more detailed physiological properties, for the analysis of antimicrobial drug-cell interactions, and for the isolation of high-yielding strains of biotechnological interest. Flow cytometric analyses provide an abundance of multivariate data, and special methods have been devised to exploit these. Ongoing advances mean that modern flow cytometers may now be used by nonspecialists to effect a renaissance in our understanding of microbial heterogeneity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.