ArticleProceedings of the National Academy of Sciences of the United States of America1996
Determination of the lifetime and force dependence of interactions of single bonds between surface-attached CD2 and CD48 adhesion molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Receptor-Ligand Binding: Effect of Mechanical Factors.International journal of molecular sciences · 2023Review
- Supported Lipid Bilayers and the Study of Two-Dimensional Binding Kinetics.Frontiers in molecular biosciences · 2022Review
- Nanoscale Functionalized Particles with Rotation-Controlled Capture in Shear Flow.ACS applied materials & interfaces · 2018Article
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- Shear stress increases the residence time of adhesion of Pseudomonas aeruginosa.Biophysical journal · 2011Article
- Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.PLoS computational biology · 2009Article
- Studying Molecular Interactions at the Single Bond Level with a Laminar Flow Chamber.Cellular and molecular bioengineering · 2008Article
- Measuring diffusion and binding kinetics by contact area FRAP.Biophysical journal · 2008Article
- Dissecting subsecond cadherin bound states reveals an efficient way for cells to achieve ultrafast probing of their environment.FEBS letters · 2007Article
- Dynamics of unbinding of cell adhesion molecules: transition from catch to slip bonds.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Effects of intermediate bound states in dynamic force spectroscopy.Biophysical journal · 2004Article
- Avidity enhancement of L-selectin bonds by flow: shear-promoted rotation of leukocytes turn labile bonds into functional tethers.The Journal of cell biology · 2003Article
- Differential segregation in a cell-cell contact interface: the dynamics of the immunological synapse.Biophysical journal · 2002Article
- Fast dissociation kinetics between individual E-cadherin fragments revealed by flow chamber analysis.The EMBO journal · 2002Article
- Dissecting streptavidin-biotin interaction with a laminar flow chamber.Biophysical journal · 2002Article
- Shear flow-induced detachment kinetics of Dictyostelium discoideum cells from solid substrate.Biophysical journal · 2002Article
- Diffusion of microspheres in shear flow near a wall: use to measure binding rates between attached molecules.Biophysical journal · 2001Article
- Cadherin interaction probed by atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Direct observation of the anchoring process during the adsorption of fibrinogen on a solid surface by force-spectroscopy mode atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Experimental study of the interaction range and association rate of surface-attached cadherin 11.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied single molecular interactions between surface-attached rat CD2, a T-lymphocyte adhesion receptor, and CD48, a CD2 ligand found on antigen-presenting cells. Spherical particles were coated with decreasing densities of CD48-CD4 chimeric molecules then driven along CD2-derivatized glass surfaces under a low hydrodynamic shear rate. Particles exhibited multiple arrests of varying duration. By analyzing the dependence of arrest frequency and duration on the surface density of CD48 sites, it was concluded that (i) arrests were generated by single molecular bonds and (ii) the initial bond dissociation rate was about 7.8 s-1. The force exerted on bonds was increased from about 11 to 22 pN; the detachment rate exhibited a twofold increase. These results agree with and extend studies on the CD2-CD48 interaction by surface plasmon resonance technology, which yielded an affinity constant of approximately 10(4) M-1 and a dissociation rate of > or = 6 s-1. It is concluded that the flow chamber technology can be an useful complement to atomic force microscopy for studying interactions between isolated biomolecules, with a resolution of about 20 ms and sensitivity of a few piconewtons. Further, this technology might be extended to actual cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.