ArticleThe Journal of clinical investigation1996
Repertoire cloning of lupus anti-DNA autoantibodies.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 83 citations in OpenAlex.
- Cytosolic Internalization of Anti-DNA Antibodies by Human Monocytes Induces Production of Pro-inflammatory Cytokines Independently of the Tripartite Motif-Containing 21 (TRIM21)-Mediated Pathway.Frontiers in immunology · 2018Article
- Protective natural autoantibodies to apoptotic cells: evidence of convergent selection of recurrent innate-like clones.Annals of the New York Academy of Sciences · 2015Review
- Wiskott-Aldrich Syndrome protein deficiency perturbs the homeostasis of B-cell compartment in humans.Journal of autoimmunity · 2014Article
- Next generation sequencing reveals skewing of the T and B cell receptor repertoires in patients with wiskott-Aldrich syndrome.Frontiers in immunology · 2014Article
- Article
- Pathogenic and Epiphenomenal Anti-DNA Antibodies in SLE.Autoimmune diseases · 2010Article
- Early diagnosis of systemic lupus erythmatosus using ANN models of dsDNA binding antibody sequence data.Bioinformation · 2010Article
- Genetic imprinting of autoantibody repertoires in systemic lupus erythematosus patients.Clinical and experimental immunology · 2008Article
- Isolation of pathogenic monoclonal anti-desmoglein 1 human antibodies by phage display of pemphigus foliaceus autoantibodies.The Journal of investigative dermatology · 2008Article
- Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models.BMC biotechnology · 2007Article
- B cells in autoimmune diseases: insights from analyses of immunoglobulin variable (Ig V) gene usage.Autoimmunity reviews · 2007Review
- The evolution of human anti-double-stranded DNA autoantibodies.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display.The Journal of clinical investigation · 2005Article
- The role of somatic mutation in determining the affinity of anti-DNA antibodies.Clinical and experimental immunology · 2003Article
- The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus.Arthritis research & therapy · 2003Article
- Perturbations in the impact of mutational activity on Vlambda genes in systemic lupus erythematosus.Arthritis research · 2001Article
- Immunoglobulin kappa chain receptor editing in systemic lupus erythematosus.The Journal of clinical investigation · 1998Article
- The human immune response to red blood cell antigens as revealed by repertoire cloning.Immunologic research · 1998Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the autoantibody repertoire associated with SLE, we have created phage display IgG Fab libraries from two clinically active SLE patients and from the healthy identical twin of one of these patients. The libraries from the lupus discordant twins were found to both include unusually large representations of the V(H)5 gene family. By panning with DNA, the SLE libraries each yielded IgG anti-double-stranded (ds) DNA autoantibodies, which are characteristic of lupus disease. These included a V(H)5 autoantibody from the affected twin, that has a targeted cluster of mutations that potentially improves binding affinity. The recovered IgG anti-dsDNA autoantibodies expressed the same idiotypes associated with the in vivo IgG anti-dsDNA response of the respective SLE donor. Heavy-light chain shuffling experiments demonstrated a case in which the in vitro creation of anti-dsDNA binding activity required restrictive pairing of a heavy chain with Vlambda light chains similar to those in circulating anti-dsDNA autoantibodies. By contrast, IgG anti-ds autoantibodies could not be recovered from the library from the healthy twin, or from shuffled libraries with heavy chains from the healthy twin. These repertoire analyses illustrate how inheritance and somatic processes interplay to produce lupus-associated IgG autoantibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.