ArticlePlant molecular biology1996
Single-site manipulation of tomato chromosomes in vitro and in vivo using Cre-lox site-specific recombination.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Development of selectable marker free, insect resistant, transgenic mustard (Brassica juncea) plants using Cre/lox mediated recombination.BMC biotechnology · 2013Article
- A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
- Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors.Plant molecular biology · 2008Article
- Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites.Plant molecular biology · 2005Article
- Utility of the FLP-FRT recombination system for genetic manipulation of rice.Plant cell reports · 2005Article
- Biochemical, genetic, and zoosporicidal properties of cyclic lipopeptide surfactants produced by Pseudomonas fluorescens.Applied and environmental microbiology · 2003Article
- Transpositional behaviour of an Ac/Ds system for reverse genetics in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Site-specific recombination for genetic engineering in plants.Plant cell reports · 2003Review
- Visualization of somatic deletions mediated by R/RS site-specific recombination and induction of germinal deletions caused by callus differentiation and regeneration in rice.Plant cell reports · 2003Article
- A self-excising Cre recombinase allows efficient recombination of multiple ectopic heterospecific lox sites in transgenic tobacco.Transgenic research · 2003Article
- Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome.Plant molecular biology · 2003Article
- FLP/FRT-mediated restoration of normal phenotypes and clonal sectors formation in rolC transgenic tobacco.Transgenic research · 2001Article
- A recombinase-mediated transcriptional induction system in transgenic plants.Plant molecular biology · 2001Article
- A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination.Nucleic acids research · 2000Article
- Cre/lox-mediated site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana by transient expression of cre.Plant molecular biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the aim of developing new techniques for physical and functional genome analysis, we have introduced the Cre-lox site-specific recombination system into the cultivated tomato (Lycopersicon esculentum). Local transposition of a Ds(lox) transposable element from a T-DNA(lox) on the long arm of chromosome 6 was used to position pairs of lox sites on different closely linked loci. In vitro Cre-lox recombination between chromosomal lox sites and synthetic lox oligonucleotides cleaved the 750 Mb tomato genome with 34 bp specificity to release unique 65 kb and 130 kb fragments of chromosome 6. Parallel in vitro experiments on Saccharomyces cerevisiae chromosomes show the efficiency of cleavage to be 50% per chromosomal lox site at maximum. By expressing the Cre recombinase in tomato under control of a constitutive CaMV 35S promoter, efficient and specific somatic and germinal in planta inversion of the 130 kb fragment is demonstrated. The combined use of in vitro and in vivo recombination on genetically mapped lox sites will provide new possibilities for long range restriction mapping and in vivo manipulation of selected tomato genome segments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.