ArticlePlant molecular biology1996
Different sequences for 5'-untranslated leaders of nuclear genes for plastid proteins affect the expression of the beta-glucuronidase gene.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
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- The 5'UTR Intron of Arabidopsis GGT1 Aminotransferase Enhances Promoter Activity by Recruiting RNA Polymerase II.Plant physiology · 2016Article
- Genome-wide conserved non-coding microsatellite (CNMS) marker-based integrative genetical genomics for quantitative dissection of seed weight in chickpea.Journal of experimental botany · 2015Article
- Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.Journal of biosciences · 2009Article
- Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif.Transgenic research · 2007Article
- Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta.Transgenic research · 2005Article
- Isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression.Current genetics · 2005Article
- The cauliflower mosaic virus 35S promoter extends into the transcribed region.Journal of virology · 2004Article
- Contribution of downstream promoter elements to transcriptional regulation of the rice tungro bacilliform virus promoter.Nucleic acids research · 2002Article
- Differential regulation of mRNA levels of acyl carrier protein isoforms in Arabidopsis.Plant physiology · 2002Article
- Differential expression of two spermidine synthase genes during early fruit development and in vegetative tissues of pea.Plant molecular biology · 1999Article
- A model for RNA-mediated gene silencing in higher plants.Plant molecular biology · 1998Review
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Expression of chimeric uidA gene fusions (for bacterial beta-glucuronidase) with 5'-flanking sequences of the spinach AtpC and PetE genes (encoding the subunit gamma of the chloroplast ATP synthase and plastocyanin, respectively) requires sequences for the 5'-untranslated leaders. The sequence for the PetE leader does not exhibit significant similarities to those of other leader sequences. Closer inspection of PetE uncovered that the crucial region is located in the vicinity of the transcription start site (+5/+15, TTGTCATTTCT). In contrast, 3' deletions of sequences for the AtpC leader revealed that the region in the vicinity of the translation initiation codon is essential for uidA gene expression (+103/+176). This segment contains a CT-rich sequence (TTCTCTCTCCT), which is found identically or in a slightly modified form in sequences for 85 plant leaders deposited in the EMBL data bank. Site-directed mutagenesis of the CT-rich sequence resulted in a three-fold reduction of the transcription of the transgene. It is concluded (1) that different elements in the sequences for the spinach PetE and AtpC leaders control the expression of the uidA gene, (2) that these elements operate transcriptionally rather than post-transcriptionally and (3) that a CT-rich sequence represents a crucial cis element for the transcription of the AtpC::uidA gene fusion.
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