ArticlePlant molecular biology1996
Characterization of replication origins flanking the 23S rRNA gene in tobacco chloroplast DNA.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Efficient Replication of the Plastid Genome Requires an Organellar Thymidine Kinase.Plant physiology · 2018Article
- Identification of a soybean chloroplast DNA replication origin-binding protein.Plant molecular biology · 2011Article
- The evolution of the plastid chromosome in land plants: gene content, gene order, gene function.Plant molecular biology · 2011Article
- Linear molecules of tobacco ptDNA end at known replication origins and additional loci.Plant molecular biology · 2006Article
- Stable transformation of the cotton plastid genome and maternal inheritance of transgenes.Plant molecular biology · 2004Article
- Replication intermediate analysis confirms that chromosomal replication origin initiates from an unusual intergenic region in Caulobacter crescentus.Nucleic acids research · 2001Article
- Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts.Journal of molecular biology · 2001Article
- Fine mapping of replication origins (ori A and ori B) in Nicotiana tabacum chloroplast DNA.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using 5' end-labeled nascent strands of tobacco chloroplast DNA (ctDNA) as a probe, replication displacement loop (D-loop) regions were identified. The strongest hybridization was observed with restriction fragments containing the rRNA genes from the inverted repeat region. Two-dimensional gel analysis of various digests of tobacco ctDNA suggested that a replication origin is located near each end of the 7.1 kb BamHI fragment containing part of the rRNA operon. Analysis of in vitro replication products indicated that templates from either of the origin regions supported replication, while the vector alone or ctDNA clones from other regions of the genome did not support in vitro replication. Sequences from both sides of the BamHI site in the rRNA spacer region were required for optimal in vitro DNA replication activity. Primer extension was used for the first time to identify the start site of DNA synthesis for the D-loop in the rRNA spacer region. The major 5' end of the D-loop was localized to the base of a stem-loop structure which contains the rRNA spacer BamHI site. Primer extension products were insensitive to both alkali and RNase treatment, suggesting that RNA primers had already been removed from the 5' end of nascent DNA. Location of an origin in the rRNA spacer region of ctDNA from tobacco, pea and Oenothera suggests that ctDNA replication origins may be conserved in higher plants.
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