ArticlePlant molecular biology1996
Molecular characterization of transketolase (EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- The Draft Genome of Kochia scoparia and the Mechanism of Glyphosate Resistance via Transposon-Mediated EPSPS Tandem Gene Duplication.Genome biology and evolution · 2019Article
- Article
- Ripening of pepper (Capsicum annuum) fruit is characterized by an enhancement of protein tyrosine nitration.Annals of botany · 2015Article
- Decreased TK activity alters growth, yield and tolerance to low temperature and low light intensity in transgenic cucumber plants.Plant cell reports · 2015Article
- Cloning, expression and characterization of sugarcane (Saccharum officinarum L.) transketolase.The protein journal · 2013Article
- Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.Open biology · 2012Article
- Development of a gene silencing DNA vector derived from a broad host range geminivirus.Plant methods · 2009Article
- Article
- Structure and properties of an engineered transketolase from maize.Plant physiology · 2003Article
- Genes for two mitochondrial ribosomal proteins in flowering plants are derived from their chloroplast or cytosolic counterparts.The Plant cell · 2002Article
- The plastidic pentose phosphate translocator represents a link between the cytosolic and the plastidic pentose phosphate pathways in plants.Plant physiology · 2002Article
- A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism.The Plant cell · 2001Article
- Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits1.Plant physiology · 1998Article
- Higher-plant chloroplast and cytosolic fructose-1,6-bisphosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence.Plant molecular biology · 1996Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding the Calvin cycle enzyme transketolase (TKL; EC 2.2.1.1) was isolated from Sorghum bicolor via subtractive differential hybridization, and used to isolate several full-length cDNA clones for this enzyme from spinach. Functional identity of the encoded mature subunit was shown by an 8.6-fold increase of TKL activity upon induction of Escherichia coli cells that overexpress the spinach TKL subunit under the control of the bacteriophage T7 promoter. Chloroplast localization of the cloned enzyme is shown by processing of the in vitro synthesized precursor upon uptake by isolated chloroplasts. Southern blot-analysis suggests that TKL is encoded by a single gene in the spinach genome. TKL proteins of both higher-plant chloroplasts and the cytosol of non-photosynthetic eukaryotes are found to be unexpectedly similar to eubacterial homologues, suggesting a possible eubacterial origin of these nuclear genes. Chloroplast TKL is the last of the demonstrably chloroplast-localized Calvin cycle enzymes to have been cloned and thus completes the isolation of gene probes for all enzymes of the pathway in higher plants.
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